Biomerieux, Inc.

Biomerieux Sa

Antoine Vimont

David Mosticone

Bruno Colin

Jean-Claude Raymond

Jean-Pierre Flandrois

Thierry Sofia

Florent Baril

Thomas Junillon

Sylvain Orenga

Marie Cellier

Yuping Guo

Edgard Minassian

John Mills

Benoît Mallen

Pascal Montes

Vincent Atrache

Bouchra Makrouf

Benoit Mallen

Aurelie Lafay

Martine Dhedin

Jean-Pierre Flandroix

Jean Claude Raymond

A method for detecting at least one microorganism that may be present in a sample, comprising the steps of:

Method for the real-time detection of microorganisms in a liquid culture medium by agglutination

The present invention generally relates to the field of analysis for example biological analysis. More specifically, the present invention relates to a process of detecting at least one microorganism present in a sample placed in a closed container, said method comprising essentially the following steps:

Process of directly detecting and identifying a microorganism in a biological sample diluted in an enrichment broth

A method of processing at least one biological sample capable of containing at least one target microorganism, the method being carried out within a container and including: (a) performing at least one step of homogenizing the biological sample, during which contents of the container are displaced from a level n, corresponding to a level of the contents at rest, to a homogenization level nh, distinct from the level n, and vice versa; and (b) generating a displacement of the contents to a level n+1, which is different from levels n and nh, such that the contents come into contact with at least one culture, at least one analysis device, or a combination thereof, positioned in a chamber of the container, between level n+1 inclusive and level nh exclusive, wherein step b) takes place before, after or during all or part of the homogenization step a).

Method for treating at least one biological sample containing a target microorganism

A method for detecting at least one microorganism present in a sample, that includes: a) in a first container, bringing the sample into contact with at least one culture medium, b) placing the first container in suitable conditions to permit growth of the microorganism or microorganisms, c) bringing some or all of the mixture being made of the sample and the culture medium into contact with a reaction mixture and a substrate for capturing the microorganism(s) in the first container or in a second container, the reaction mixture having a device for detecting the microorganism(s); d) detecting, within the first or second container, the presence of the microorganism or microorganisms detected by the detecting device and fixed on the capture substrate.

Method for detecting and directly identifying a microorganism in a biological sample by an optical route

One embodiment provides a device for processing at least one biological sample capable of containing at least one target microorganism within at least one container. The device having at least one displacement device for generating the displacement of the contents of the at least one container and at least one site for receiving the at least one container. Additionally, the at least one container can receive the at least one biological sample within the at least one container, the container being delimited by a wall fixed on a base. Further, the at least one displacement device may be movable with respect to the base, and the at least one container may include a flexible material which allows the at least one container to be compressed against said wall.

The present invention relates to the field of microbiological testing of food. It relates to a kit comprising a reaction medium containing at least one inhibitor of Gram-negative bacteria and a fluorescent substrate specific for PC-PLC. It further relates to a diagnostic kit for identifying bacteria of thegroup comprising a container of a selective or nonselective reaction medium with a pH between 6.8 and 8.0, the medium comprising at least one inhibitor of Gram-negative bacteria and a fluorescent phosphatidylcholine phospholipase C (PC-PLC) substrate.

[object Object]

The present invention relates to a method for detecting at least one target microorganism that may be present in a sample, comprising the steps of: The present invention also relates to a method for detecting and identifying at least one target microorganism that may be present in a sample.

Method for real-time detection of microorganisms in a liquid culture medium using cellular lysis

The present invention relates to a method for identifying bacteria of thegroup, comprising the following steps: (a) providing a sample that may contain bacteria of thegroup, a reaction medium comprising at least one fluorescent phosphatidylcholine phospholipase C (PC-PLC) substrate and an inhibitor of Gram-negative bacteria; (b) inoculating the reaction medium with the sample; (c) incubating the inoculated reaction medium; and (d) identifying the bacteria of thegroup by detecting the PC-PLC substrate hydrolysis reaction, in which the pH of the reaction medium and the time necessary for detecting the PC-PLC substrate hydrolysis reaction are adapted such that said hydrolysis reaction by bacteria of thegroup is detected before hydrolysis of the PC-PLC substrate by any Gram-positive bacteria other than those belonging to thegroup, that may be present in the sample.

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Lyons, France

Antoine Vimont