Commissariat &#xc0; L'&#xc9;nergie Atomique Et Aux &#xc9;nergies Alternatives

Commissariat a L'energie Atomique

Université Joseph Fourier

Azbil Corporation

Antoine Hoang

Françoise Vinet

Isabelle Texier-Nogues

Guillaume Delapierre

Vincent Agache

Franck Martin

Aude Bernardin

Pascal Dumy

Séverine Vignoud

Véronique Robert

Eric Defrancq

Christophe Gaude

Audrey Le Naour

The present invention relates to a method for functionalizing fluid lines () in a micromechanical device, the walls of which include an opaque layer. For this purpose, the invention provides a method for functionalizing a micromechanical device provided with a fluid line including a peripheral wall () having a surface () outside the line and an inner surface () defining a space () in which a fluid can circulate, the peripheral wall at least partially including a silicon layer (). The method includes the following steps: a) providing a device, the peripheral wall () of which at least partially includes a silicon layer () having, at least locally, a thickness (e) of more than 100 nm and less than 200 nm, advantageously of 160 to 180 nm; c) silanizing at least the inner surface of the fluid line; d) the localized, selective photo-deprotection on at least the inner surface of the silanized device by exposing the peripheral wall () at the point at which said wall has a thickness (e) of more than 100 nm and less than 200 nm, advantageously of 160 to 180 nm.

Method for functionalising fluid lines contained in a micromechanical device, micromechanical device including functionalised lines, and method for manufacturing same

The invention relates to a silane compound including a cycloalkyne functionality, to a method for functionalising a solid substrate, and to the solid substrate thus produced. The silane compound of the invention corresponds to the formula X-E-A-Z where X is a silyl group, E is an organic spacer group, A is a single bond or a &#x2014;CONH&#x2014;, &#x2014;NHCO&#x2014;, &#x2014;OCHCONH&#x2014;, &#x2014;NHCOCHO&#x2014;, &#x2014;O&#x2014; or &#x2014;S&#x2014; group, and Z is a cycloalkyne or heterocycloalkyne with at least 8 members. The invention is particularly suitable for use in the field of medicine.

Silane compounds having a cyclic carbon chain including an alkyne function for functionalizing solid substrates and immobilizing biological molecules on said substrates

The invention relates to novel silane compounds corresponding to the following formula (I): in which Rcan represent a methyl group, Rand Rcan represent a hydrogen atom, A can represent &#x2014;O&#x2014;, E can represent an alkylene group, X can represent a methoxysilane group, Z can represent a simple bond and Y can represent an &#x2014;Nor &#x2014;N&#x2014;NHgroup. Use of these silane compounds to functionalize solid supports and to immobilize biological molecules on these supports.

Silane compounds carrying a hydrazone or diazo functional group in order to functionalize solid supports and immobilize biological molecules on these supports

The invention relates to compounds comprising a photolabile protecting group and to the use thereof as coupling agents for the functionalisation of solid supports. The invention also relates to the solid supports functionalised by said compounds and to the use of same for the immobilisation of biological molecules of interest, such as nucleic acid molecules.

Coupling agents comprising a photolabile protecting group and uses thereof, such as for the functionalisation of solid supports

Growing community of inventors

Grenoble, France

Antoine Hoang