Genencor International, Inc.

Genecor International, Inc.

Solvay Enzymes Gmbh & Co. Kg

Kali-chemie Aktiengesellschaft

Gesellschaft Fuer Biotechnologische Forschung Mbh

Antoine Amory

Philippe Deweer

Roman Vetter

Gerhard Konieczny-Janda

Andre Clippe

Pierre Ledoux

Eric De Buyl

Christophe Andre

Ren&eacute; Detroz

Andr&eacute;e Lahaye

Huaming Wang

Detlef Wilke

Wolfgang Aehle

Dietmar Schomburg

Hubert A Herrmann

Cynthia C Wang

Patrick Dhaese

Karsten B&ouml;rner

Annick Lambrechts-Rongvaux

Andr&eacute; Clippe

Disclosed herein are phenol oxidizing enzymes obtainable from species ofwhich are useful in modifying the color associated with dyes and colored compounds, as well as in anti-dye transfer applications. Also disclosed herein are biologically-pure cultures of strains of the genus, designated hereinMUCL 38996 andMUCL 38898, which are capable of naturally-producing the novel phenol oxidizing enzymes. Disclosed herein is the amino acid and nucleic acid sequence forphenol oxidizing enzymes as well as expression vectors and host cells comprising the nucleic acid. Disclosed herein are methods for producing the phenol oxidizing enzyme as well as methods for constructing expression host. Disclosed herein are enzyme compositions comprising phenol oxidizing enzymes obtainable from species of

Oxidizing enzymes

A purified xylanase derived from PRL B12 is disclosed. This xylanase is efficient for use in the biobleaching of wood pulp, permitting a strong reduction in the quantity of chlorine used and AOX compounds produced in classical and ECF wood pulp bleaching sequences as well as the quantity of ozone used in TCF sequences. The gene coding for the xylanase was isolated and purified and used to construct an expression vector therefor. A recombinant host strain of is also disclosed which is efficient for expressing heterologous enzymes, including the xylanase when transformed by the expression vector.

Xylanase derived from a Bacillus species, expression vectors for such xylanase and other proteins, host organisms therefor and use thereof

Xylanase derived from a bacillus species, expression vectors for such xylanase and other proteins, host organisms therefor and use thereof

The invention relates to a novel high-alkaline protease, its use in the industrial or domestic fields and also compositions for said uses containing this protease. The protease of the invention is a selected triple mutant derived from certain precursor proteases from the subtilisin group, especially of the &ldquo;subtilisin 309&rdquo; type.

High-alkaline protease and its use arginine-substituted subtilisin composition and use

The use of alkaline bacillus proteases in commercial laundry methods and compositions containing these proteases for commercial laundering are described.

Use of alkaline proteases in industrial textile laundering processes

The invention relates to a heat-stable pullulanase having the property of hydrolysing glucosidic bonds of the .alpha.-1,6 type in amylopectin and having an enzymatic activity in an acid medium and at a temperature of about 60.degree. C. The invention also relates to strains of microorganisms which produce this pullulanase and processes for the preparation of this pullulanase. The invention also relates to the uses thereof and compositions comprising the product. The invention also relates to a DNA molecule. The invention relates to an expression vector containing this DNA molecule and to a chromosomal integration vector containing this DNA molecule.

Pullulanase, microorganisms which produce it, processes for the

Pullulanase producing microrganisms

Expression system for novel pullulanase

The invention relates to a heat-stable pullulanase having the property of hydrolysing glucoside bonds of the .alpha.-1,6 type in amylopectin and having an enzymatic activity in an acid medium and at a temperature of about 60.degree. C. The invention also relates to strains of microorganisms which produce this pullulanase and processes for the preparation of this pullulanase. The invention also relates to the uses thereof and compositions comprising the product. The invention also relates to a DNA molecule. The invention relates to an expression vector containing this DNA molecule and to a chromosomal integration vector containing this DNA molecule.

Process for the saccharification of starch

The invention relates to a heat-stable pullulanase having the property of hydrolysing glucosidic bonds of the .alpha.-1,6 type in amylopectin and having an enzymatic activity in an acid medium and at a temperature of about 60.degree. C. The invention also relates to strains of micro-organisms which produce this pullulanase and processes for the preparation of this pullulanase. The invention also relates to the uses thereof and compositions comprising the product. The invention also relates to a DNA molecule. The invention relates to an expression vector containing this DNA molecule and to a chromosomal integration vector containing this DNA molecule.

Process for the production of novel pullulanase

The invention relates to a heat-stable pullulanase having the property of hydrolysing glucosidic bonds of the .alpha.- 1,6 type in amylopectin and having an enzymatic activity in an acid medium and at a temperature of about 60.degree. C. The invention also relates to strains of microorganisms which produce this pullulanase and processes for the preparation of this pullulanase. The invention also relates to the uses thereof and compositions comprising the product. The invention also relates to a DNA molecule. The invention relates to an expression vector containing this DNA molecule and to a chromosomal integration vector containing this DNA molecule.

Pullulanase

Novel, optimized highly alkaline proteases which are suitable for use in detergent formulations are prepared by employing microorganisms transformed by mutated DNA sequences. The mutated sequences are obtained starting from DNA sequences which code for highly alkaline protease usually produced by Bacillus species by altering these DNA sequences in defined positions by directed mutagenesis (point mutation) in such a way that the codon in which the point mutation is located now codes for an amino acid which is more strongly basic than the original amino acid. The result is highly alkaline proteases in which original amino acids have been replaced by more strongly basic amino acids, preferably by the amino acids lysine or arginine. Synthetic oligonucleotides, DNA sequences, vectors and transformed microorganisms which are used for generating and obtaining the optimized highly alkaline protease are also described.

Highly alkaline proteases

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Rixensart, Belgium

Antoine Amory