Biospyder Technologies, Inc.

Utah Ventures Ii, L.p.

Novadx, Inc.

Anthony Stevens

Bruce Seligmann

Joanne M Yeakley

Joel McComb

Paul Roben

Ligation assays in liquid phase for detecting nucleic acid sequences.

Ligation assays in liquid phase

This invention provides novel methods of and kits for labeling and isolating lumen-exposed molecules, particularly polypeptides which are expressed in a tissue-specific or organ-specific manner. The methods and kits can be used to isolate molecules exposed on the luminal side of cells lining vascular, ductal, sinus, respiratory, fascial, and other perfusible tissue spaces.

Methods for identifying and isolating tissue-specific lumen-exposed molecules

A method for specifically immunoprecipitating albumin from a serum sample, using a &ldquo;collapsible affinity matrix.&rdquo; Also provided is a method for the co-removal of immunoglobulin using a &ldquo;collapsible affinity matrix.&rdquo; Removal of the highly abundant serum proteins, albumin and immunoglobulin, thereby improves the fractionation of the remaining serum proteins. Due to the collapsible nature of the matrix, less protein is trapped in the void space. Through specific removal of the abundant serum proteins by the collapsible affinity matrix and application of a two dimensional gel electrophoresis method, HiCap 2-D PAGE, the concentrations of a large number of low abundant serum proteins are estimated simultaneously, allowing the identification of several disease-related proteins in a relatively short period of time.

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San Diego, CA, United States of America

Anthony Stevens