Tularik Limited

Anthony Simon Lynch

Binoj Joseph Matthew

Mohanram Sivaraja

Michael Gregory Peterson

Ashok Ramesh Sanadi

This invention provides novel assays for topoisomerase activity and for identifying topoisomerase inhibitors. The assays include both solid-phase and liquid-phase methods that are amenable to high throughput screening methods. The assays of the invention are readily adaptable to numerous types of topoisomerase, and are capable of identifying novel classes of topoisomerase activity modulators.

High-throughput screening assays for modulators of nucleic acid topoisomerases

Screening assays for identifying ligase activity modulators are provided, in both solid phase and liquid phase formats. Solid phase formats detect ligase activity by ligating a labelled nucleic acid to a capture nucleic acid in the presence of the ligase modulator and detection of the labelled nucleic acid. Liquid phase assays detect ligation-dependent changes in interactive labels between nucleic acids such as proximity quenching of fluorescent labels. Compositions, apparatus and integrated systems for assays are also provided.

High throughput screening assays for nucleic acid ligase modulators

High-throughput screening assays for modulators of nucleic acid

High-throughput in vitro assays are provided for identifying modulators of peptidyl transferase. New solid-phase assays, related compositions, apparatus and integrated systems are provided.

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Pacifica, CA, United States of America

Anthony Simon Lynch