Quest Diagnostics Investments, Inc.

Anthony Sferruzza

Kevin Z Qu

Ke Zhang

Renius Owen

Yan Liu

Provided herein are methods for miRNA profiling for the diagnosis, prognosis, and management of melanoma and differentiation of melanoma from nevi.

MicroRNA profiling for diagnosis of dysplastic nevi and melanoma

Microrna profiling for diagnosis of dysplastic nevi and melanoma

The present invention provides methods for determining the genotype of a selected gene present in at least two alleles in a sample. The methods involve amplifying DNA from the sample with a first pair of flanking primers that hybridize to nucleic acid sequences flanking a variant-specific gene sequence, the presence of which indicates the presence of a first gene variant, and the absence of which indicates the presence of a second gene variant. The DNA is also amplified with a third primer that specifically binds to the variant-specific sequence and together with one of the flanking primers forms a second pair of primers. Detection of one or more nucleic acid products of the amplification reaction is indicative of the genotype present in the sample.

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San Clemente, CA, United States of America

Anthony Sferruzza