Danisco US Inc.

The Goodyear & Tire Rubber Company

Butamax Advanced Biofuels LLC

Anthony R Calabria

Michael C Miller

Gopal K Chotani

Karl John Sanford

Joseph Charles McAuliffe

Gregory Marshall Whited

Marguerite A Cervin

Richard La Duca

Timothy A Sabo

Alex Toftgaard Nielsen

Erin L Spring

Zachary Quinn Beck

Dmitrii V Vaviline

Jayarama K Shetty

Joseph J Zaher

Vivek Sharma

Sung Ho Lee

William Albert Cuevas

Keith H Burlew

Gang Duan

Paula Johanna Maria Teunissen

Benjamin Fuchs

Bruce A Strohm

Robin Fong

Ying Qian

Adam Bernfeld

Hongxian Xu

Duncan Coffey

Martien H Bergsma

Ana Paula Goes

Erin L Webster

Adam David Henry

Walter Tamulis

The invention features methods for producing isoprene from cultured cells wherein the cells in the stationary phase. The invention also provides compositions that include these cultured cells and/or increased amount of isoprene. The invention also provides for systems that include a non-flammable concentration of isoprene in the gas phase. Additionally, the invention provides isoprene compositions, such as compositions with increased amount of isoprene or increased purity.

Compositions and methods for producing isoprene free of c5 hydrocarbons under decoupling conditions and/or safe operating ranges

The present disclosure is directed to methods, approaches, devices, equipment, and systems for minimizing or reducing contamination in facilities implementing fermentation or distillation processes. In embodiments, the facility is a biofuel plant that produces fermentation product such as product alcohol like butanol. In some embodiments, the methods, approaches, devices, equipment, and systems are operable to implement clean in place contamination (CIP) mitigation techniques that can also include sterilize in place (SIP) mitigation techniques to decontaminate equipment including surfaces of the equipment that come in contact with materials used in the production of product alcohols. Other cleaning and contamination minimizing techniques are also described.

Contamination mitigation

Compositions and methods for producing isoprene free of C5 hydrocarbons under decoupling conditions and/or safe operating ranges

The invention features methods for producing isoprene from cultured cells using a feedback-resistant mevalonate kinase polypeptide, such as an archaeal mevalonate kinase polypeptide. The resulting isoprene compositions may have increased yields and/or purity of isoprene.

Increased isoprene production using the archaeal lower mevalonate pathway

The invention features methods for producing isoprene from cultured cells wherein the cells in the stationary phase. The invention also provides compositions that include these cultured cells and/or increased amount of isoprene. The invention also provides for systems that include a nonflammable concentration of isoprene in the gas phase. Additionally, the invention provides isoprene compositions, such as compositions with increased amount of isoprene or increased purity.

Embodiments of the present disclosure relate to a process for producing isoprenoid precursor molecules and/or isoprenoids from a starch substrate by saccharification and/or fermentation. The saccharification is effectively catalyzed by a glucoamylase at a pH in the range of 5.0 to 8.0. At a pH of 6.0 or above, the glucoamylase possesses at least 50% activity relative to its maximum activity. The saccharification and fermentation may be performed as a simultaneous saccharification and fermentation (SSF) process.

Production of isoprenoids under neutral pH conditions

The invention provides improved methods for the production of isoprene from biological materials using a membrane bioreactor to culture isoprene-producing cells.

Methods of producing isoprene comprising a membrane bioreactor

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Wilmington, DE, United States of America

Anthony R Calabria