10X Genomics, Inc.

Bio-Rad Laboratories, Inc.

Lawrence Livermore National Security, LLC

Anthony Makarewicz

Rajiv Bharadwaj

Bill Kengli Lin

Christopher Hindson

Kevin D Ness

Benjamin J Hindson

Michael Schnall-Levin

Donald Masquelier

Serge Saxonov

Debkishore Mitra

Tobias Daniel Wheeler

Mohammad Rahimi Lenji

Pranav Patel

Andrew D Price

Amy L Hiddessen

Yifeng Yin

Luc J Bousse

Stefano Schiaffino

Katherine Pfeiffer

Zahra Kamila Belhocine

Steven W Short

John Dzenitis

William J Benett

David P Stumbo

Satinderpall Singh Pannu

Dmitri Simonian

Joshua T Oen

Denis Pristinski

Kamila Belhocine

Vincent Riot

Nicholas Heredia

Erin Rae Chia

David Glade

George Carman

Steve Hobbs

Dean R Hadley

Bruce Henderer

John T Andreski

Claudia K Hertzog

Methods and systems for sorting particles are provided. Methods and systems for sorting cell beads are provided. In some cases, cell beads may be sorted from particles unoccupied with cell derivatives. In some cases, singularly occupied cell beads may be sorted from unoccupied particles and multiply occupied cell beads.

Methods for sorting particles

Methods and systems are provided for sample preparation techniques and sequencing of macromolecular constituents of cells and other biological materials.

Methods and systems for analysis

Methods and systems for droplet-based single cell barcoding

Devices, systems, and their methods of use, for generating droplets are provided. One or more geometric parameters of a microfluidic channel can be selected to generate droplets of a desired and predictable droplet size.

Droplet forming devices and system with differential surface properties

The disclosure provides devices, systems and methods for the generation of encapsulated reagents and the partitioning of encapsulated reagents for use in subsequent analyses and/or processing, such as in the field of biological analyses and characterization.

Fluidic devices, systems, and methods for encapsulating and partitioning reagents, and applications of same

Provided are microfluidics systems, devices, and networks for generating partitions. The microfluidics systems, devices, and networks may facilitate efficient merging of one or more channels in a microfluidic network and prevent blockage of a channel segment, such as by gas (e.g., air) bubbles or by one or more particles in a fluid.

Microfluidic channel networks for partitioning

Systems and methods for detection of a signal from droplets of an emulsion. An exemplary system may comprise a fluid transporter having a tube with an open end for aspirating droplets, a singulator to arrange the droplets in single file and to space the single-file droplets from one another, and a detection channel in optical communication with a detector configured to detect a signal from droplets. In some embodiments, the singulator may have a channel junction at which a stream of droplets in single file is combined with a stream of spacing fluid, and a tapered spacing channel extending downstream from the channel junction toward the detection channel. In some embodiments, the fluid transporter may suck droplet-containing fluid and spacing fluid through the detection channel from respective sources. In some embodiments, droplets may be subjected to a disaggregation routine before they are passed through the detection channel.

System and method for droplet detection

Method of producing emulsions

Devices, systems, and kits for forming droplets

Devices having a plurality of droplet formation regions

Microfluidic system, including methods and apparatus, for processing fluid, such as by droplet generation. In an exemplary method, a sample-containing fluid may be dispensed into a well through a sample port of a channel component. The channel component may include (a) a body having a bottom surface attached to the well, and a top surface with a microchannel formed therein, and (b) an input tube projecting into the well from the bottom surface of the body. The sample-containing fluid when dispensed may contact a bottom end of the input tube and may be retained, with assistance from gravity, out of contact with the microchannel. A pressure differential may be created that drives at least a portion of the sample-containing fluid from the well via the input tube and through the microchannel.

Microfluidic system with fluid pickups

Growing community of inventors

Livermore, CA, United States of America

Anthony Makarewicz