Cold Spring Harbor Laboratory

Nationaluniversity of Ireland, Maynooth

Anthony M Zador

Barak Avrum Pearlmutter

Ian D Peikon

Petr Znamenskiy

The present invention provides a method of identifying mRNA transcripts in the transcriptome of a cell comprising i) delivering into the cell a donor expression vector comprising nucleotides in a sequence encoding a trans-splicing barcode cassette, ii) exposing the cell to conditions such that the cell produces multiple copies of the trans-splicing barcode cassette encoded by the donor expression vector, which multiple copies of the trans-splicing barcode cassette each splice the barcode polynucleotide onto a mRNA transcript of the cell, thereby forming multiple mRNA transcripts of the cell, each spliced to the barcode polynucleotide; and iii) identifying the multiple mRNA transcripts that are spliced to the barcode polynucleotides, thereby identifying mRNA transcripts in the transcriptome of the cell.

Trans-splicing transcriptome profiling

Systems and methods for performing source separation are provided. Source separation is performed using a composite signal and a signal dictionary. The composite signal is a mixture of sources received by a sensor. The signal dictionary is a database of filtered basis functions that are formed by the application of directional filters. The directional filters approximate how a particular source will be received by the sensor when the source originates from a particular location. Each source can be characterized by a coefficient and a filtered basis function. The coefficients are unknown when the sources are received by the sensor, but can be estimated using the composite signal and the signal dictionary. Various ones of the sources may be selectively reconstructed or separated using the estimated value of the coefficients.

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New York, NY, United States of America

Anthony M Zador