Targeted Genetics Corporation

Anthony M Stepan

Haim Burstein

James Maurice Allen

Stephen D Lupton

Dara H Lockert

Carmel M Lynch

Tineka J Quinton

High-efficiency AAV packaging constructs and methods for their use are provided. in the present invention. These high-efficiency packaging constructs comprise an activating element (such as the P1 sequence located within the AAV S1 integration site of human chromosome 19) amplifiably linked to one or more AAV packaging genes. The constructs may be either integrated into a mammalian cell genome or maintained episomally. Use of the high-efficiency AAV packaging vectors of the invention provides for controlled amplifiable production of rAAV vector constructs.

Amplifiable adeno-associated virus(AAV) packaging cassettes for the production of recombinant AAV vectors

The present invention provides AAV &ldquo;split-packaging&rdquo; genes, and packaging cells comprising such genes, for use in the production of high titers of replication-incompetent recombinant AAV vectors that can be used to deliver transgenes of interest to a variety of mammalian cells.

AAV split-packaging genes and cell lines comprising such genes for use in the production of recombinant AAV vectors

The present invention provides recombinant adeno-associated virus (rAAV) vectors encoding a tumor necrosis factor (TNF) antagonist and methods using these vectors to reduce levels of TNF in a mammal. The invention also provides methods of using these rAAV vectors in palliating TNF-associated disorders.

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Seattle, WA, United States of America

Anthony M Stepan