Cytocell Limited

British Biocell International Limited

Anthony Lee Weston

Xin Lingyun

Donald L N Cardy

Susan D Wharam

Rene Assenberg

Peter Marsh

Trevor D Ray

Graham A Mock

Donald Leonard Nicholas Cardy

John Scott Lloyd

Wired doorbell

Battery doorbell

Contact sensor

Dongle

Chime

Motion sensor

Leak sensor

Disclosed is a complex formed by a hybridization reaction comprising four nucleic acid molecules; the complex comprising a target nucleic acid molecule and first, second and third nucleic acid probe molecules; wherein the first probe comprises a foot region which is complementary to a first portion of the target and is hybridized thereto, and an arm region which is substantially non-complementary to the target; the second probe comprises a foot region which is complementary to a second portion of the target, such that the foot region of the second probe is hybridized to the target adjacent or substantially adjacent to the foot region of the first probe, the second probe also comprising an arm region which is substantially non-complementary to the target but which is complementary and hybridized to the arm region of the first probe; the third probe being complementary, at least in part, to a portion of the arm region of the first probe, such that third probe is hybridized to the arm region of the first probe adjacent or substantially adjacent to the second probe; and wherein formation of the complex creates a functional double-stranded RNA polymerase promoter, one strand of the promoter being provided by the first probe, and the other strand being provided jointly by the second probe and by the third probe; and a method of detecting a target nucleic acid sequence of interest which method involves the formation of the complex.

Method for detecting nucleic acid target sequences involving in vitro transcription from an RNA polymerase promoter

Disclosed is a method of detecting a nucleic acid sequence of interest in a sample, the method comprising contacting the sample with first and second probes; the first probe comprising a portion complementary to the sequence of interest and so capable of hybridising thereto, and a portion non-complementary to the sequence of interest; the second probe comprising a portion complementary to the sequence of interest and so capable of hybridising thereto, and a portion non-complementary to the sequence of interest but complementary to that portion of the first probe which is non-complementary to the sequence of interest, such that the first and second probes are capable of hybridising to the sequence of interest in an adjacent or substantially adjacent manner, so as to allow complementary portions of the first and second probes to hybridize to each other; causing extension of the first probe with a nucleic acid polymerase, using the second probe as a template; and detecting directly or indirectly the extension of the first probe, so as to indicate the presence of the sequence of interest; characterised in that the first and/or second probe comprises a destabilizing moiety which cannot base pair with the reciprocal probe, thereby preventing hybridisation of the first and second probes in the absence of the sequence of interest.

Modified nucleic acid probes and use thereof

Nucleic acid promoters

Growing community of inventors

London, United Kingdom

Anthony Lee Weston