British Columbia Cancer Agency Branch

Britisg Columbia Cancer Agency Branch

Anthony Lee

Haishan Zeng

Stephen C-t Lam

David I McLean

Harvey Lui

Calum Macaulay

Pierre Lane

Hequn Wang

Hamid Pahlevaninezhad

Shuo Tang

David Mclean

A fiber-based polarization sensitive optical coherence tomography (PS-OCT) system uses a new polarization diversity detection (PDD) scheme and requires no active polarization modulating components. Retardation of the sample can be determined from amplitudes of arbitrarily-oriented x- and y-components of the reflected light. A hybrid custom 50/50 coupler with single-mode fiber inputs and polarization maintaining (PM) fiber outputs combines light from sample and reference arms of an interferometer. Another embodiment provides a system adapted to provide co-registered autofluorescence-optical coherence tomography (AF-OCT) imaging. AF excitation light is introduced and collected AF light is extracted at a fiber optic rotary joint (FORJ) equipped with an embedded dichroic mirror. A probe tip that uses a clad fiber to supply light to a focusing element provides enhanced OCT and AF performance.

Systems for optical imaging of biological tissues

A multiphoton microscope is provided. The microscope includes: an excitation source for providing an optical excitation beam at an excitation wavelength &#x3bb;; a scanner for scanning the excitation beam on a sample; an objective for irradiating the sample with the excitation beam scanned by the scanner and for collecting an emission beam from the sample; a first detector for detecting a plurality of multiphoton signals; and an emission light path allowing transmission from the objective to the first detector a wavelength band limited to greater than or equal to &#x3bb;/2 and less than &#x3bb;, wherein the plurality of multiphoton signals have wavelengths within the wavelength band; wherein the plurality of multiphoton signals com-prises a first multiphoton signal and a second multiphoton signal of different types. Fast image capture rate multiphoton microscopes for in vivo imaging, as well as photothermolysis methods using the microscopes are also provided.

Apparatus and methods for multiphoton microscopy

Growing community of inventors

Vancouver, Canada

Anthony Lee