Oxoid Limited

Radiometer Corporate Development Ltd.

Anthony Hugh Carr

Robert Andrew Badley

Thomas J Sands

Roy Holbrook

Judith Marjorie Anderson

Simon Edward Jackson

Ian Jobling

Kenneth Cherry

Method and device for monitoring changes in an array of sensor

Containers

A test for microbial growth is carried out by culturing a microorgnism to be tested in a lliquid culture medium containing an enzymatically hydrolyzable fluorogenic substrate for a period of less than about 7 hours, measuring fluorescence of the culture medium resulting from hydrolysis of the fluorogenic substrate by growth of the microorganism, continuing culturing of the microorganism in the culture medium for an additional period, and measuring microorganism growth by a non-fluorescent method as a confirmatory test of growth determined from fluorescence. Preferably, the additional culture period is overnight and the non-fluorescent method is by visual inspection. The culture medium may be in the wall of a microtitre plate, and the culture medium can contain an antibiotic to test for sensitivity of a microorganism to the antibiotic or to test for minimum inhibitory concentration of the antibiotic.

Microorganism testing with a hydrolyzable fluorogenic substrate

Growing community of inventors

Bedford, United Kingdom

Anthony Hugh Carr