Corning Incorporated

Anthony Glenn Frutos

David Henry

Ye Fang

Joydeep Lahiri

Corinne Walerack

Elizabeth Tran

David H Randle

Peter J Kalal

Steven J Jonas

Jacques Gollier

Garrett Andrew Piech

Santona Pal

Mark Alejandro Quesada

Gregory Roger Martin

Allison Jean Tanner

Ann MeeJin Ferrie

Eric John Mozdy

Vasiliy Nikolaevich Goral

Mark Francis Krol

Po Ki Yuen

Thomas Moore

David Andrew Pastel

Michael Brian Webb

Norman Henry Fontaine

Stephen Joseph Caracci

Jinlin Peng

Christie Leigh McCarthy

Gordon M Shedd

Todd M Upton

Timothy James Wood

Chuan-Che Wang

John Wilfred Coddaire

Maryanne De Chambeau

James Thomas Eickmann

Paula Mary Flaherty

Angela Langer Julien

Marshall Jay Kosovsky

John Forrest Roush

John Shyu

Tora Ann-Beatrice Eline Sirkka

Kimberly Ann Titus

Brent Ravaughn Lanterman

Volker H O Eckelt

Stephen J Carracci

A connected ecosystem for a laboratory environment comprises an electronic lab notebook, and instrumented biosafety cabinet, and one or more sensing vessels containing cell cultures. The electronic lab notebook interfaces with the instrumented biosafety cabinet to provide instructions, guidance, and monitoring of a user during the set up of the experimental protocol and to receive commands from the user via one of several input modalities. After the experimental protocol has been set up in the instrumented biosafety cabinet, cell cultures may be moved to an incubator where the connected ecosystem may provide automatic monitoring of the cultures. The automatic monitoring is provided by sensors integrated into cell culture vessels and supplemented by images of the cell cultures captured by a camera. The user may be informed of deviations from expected results detected based on the automatic monitoring.

Connected ecosystem for laboratory environment

A method for coating a surface of a cell culture article includes dissolving a polymer having a covalently attached polypeptide in an aqueous solution to produce a polymer solution. The polymer is formed from monomers selected to form a polymer having a linear backbone, wherein the polymer is crosslink free. The weight percentage of the polypeptide relative to the polymer conjugated to the polypeptide is sufficiently high to render the polymer conjugated to the polypeptide water soluble. The aqueous solution is substantially free of organic solvents. The method further includes (i) disposing the polymer solution on the surface of the cell culture article to produce a coated article; and (ii) subjecting the coated article to sufficient heat or electromagnetic radiation to attach the polymer conjugated to a polypeptide to the surface of the cell culture article.

Synthetic coating for cell culture

A method for hit compound identification in a high throughput, label-free biosensor cellular assay, one method including, for example:

High-throughput high-information content label-free cell biology screening methods

A method and apparatus, as defined herein, for use in compound screening, compound profiling, or both assays, for example, against two different cellular targets in, for example, a single cell-type.

Dual-target biosensor cell assays

The present invention overcomes the problems and disadvantages associated with prior art arrays by providing an array comprising a plurality of biological membrane microspots associated with a surface of a substrate that can be produced, used and stored, not in an aqueous environment, but in an environment exposed to air under ambient or controlled humidities. Preferably, the biological membrane microspots comprise a membrane bound protein. Most preferably, the membrane bound protein is a G-protein coupled receptor, an ion channel, a receptor serine/threonine kinase or a receptor tyrosine kinase.

Arrays of biological membranes and methods and use thereof

Described herein are supports for assaying an analyte and methods of making and using thereof.

Supports for assaying analytes and methods of making and using thereof

A screening system and method are described herein which provide a unique and practical solution for enabling label-free high throughput screening (HTS) to aid in the discovery of new drugs. In one embodiment, the screening system enables direct binding assays to be performed in which a biomolecular interaction of a chemical compound (drug candidate) with a biomolecule (therapeutic target) can be detected using assay volumes and concentrations that are compatible with the current practices of HTS in the pharmaceutical industry. The screening system also enables the detection of bio-chemical interactions that occur in the wells of a microplate which incorporates biosensors and surface chemistry to immobilize the therapeutic target at the surface of the biosensors. The screening system also includes fluid handling and plate handling devices to help perform automated HTS assays.

Label-free high throughput biomolecular screening system and method

The present invention includes several methods for modifying the current processes of manufacturing optical sensing microplates that use continuous waveguide films to reduce/eliminate crosstalk between the biosensors that are incorporated within wells. The methods include (1) physically deteriorating/removing the waveguide film between individual biosensors; (2) chemically depositing highly absorbing materials within the waveguide film between individual biosensors; (3) patterning disordered (scattering) regions between the diffraction gratings that define individual biosensors; (4) using a specific mask and depositing individual patches of waveguide film, where each patch defines at least one biosensor. Each of these methods and several other methods described herein prevent the propagation of light between individual sensing regions, thereby eliminating optical crosstalk between the biosensors. The present invention also includes the resulting microplate.

Method for eliminating crosstalk between waveguide grating-based biosensors located in a microplate and the resulting microplate

A support for performing an assay, including: a substrate having a pre-blocked binding polymer directly or indirectly attached to the substrate, the pre-blocked binding polymer having a plurality of maleic anhydride reactive groups capable of attaching to a biomolecule and a plurality of ionizable groups, the ratio of maleic anhydride reactive groups to ionizable groups is from 0.5 to 10, and the pre-blocked binding polymer does not contain a photoreactive group.

An optical reader system is described herein that uses a scanned optical beam to interrogate a biosensor to determine if a biomolecular binding event occurred on a surface of the biosensor. In one embodiment, the optical reader system includes a light source, a detector and a processor (e.g., computer, DSP). The light source outputs an optical beam which is scanned across a moving biosensor and while this is happening the detector collects the optical beam which is reflected from the biosensor. The computer processes the collected optical beam and records the resulting raw spectral or angle data which is a function of a position (and possibly time) on the biosensor. The processor can then analyze the raw data to create a spatial map of resonant wavelength (peak position) or resonant angle which indicates whether or not a biomolecular binding event occurred on the biosensor. Several other uses of the raw data are also described herein.

Spatially scanned optical reader system and method for using same

Growing community of inventors

Painted Post, NY, United States of America

Anthony Glenn Frutos