Wisconsin Alumni Research Foundation

Anthony G Frutos

Robert M Corn

Jennifer M Brockman

Disclosed is a process to construct multi-component biomolecule or cellular arrays suitable for use in SPR imaging studies of large molecule, cellular/molecular, and cell/cell interactions. Also disclosed are the resulting arrays. The success of the procedure hinges on the use of a reversible protecting group to modify reversibly &#x3c9;-functionalized alkanethiols self-assembled on metal substrates. The arrays themselves include a metal substrate, a continuous layer of an identical &#x3c9;-modified alkanthiol adhered to the metal substrate, and one or more discrete spots of biomolecules or cells directly bonded to the continuous layer of &#x3c9;-modified alkenthiol. The areas of the continuous layer of &#x3c9;-modified alkenthiol not covered by one of the discrete spots are covered by a background material resistant to non-specific protein binding.

Biomolecule and/or cellular arrays on metal surfaces

Disclosed is a process to construct multi-component biomolecule or cellular arrays suitable for use in SPR imaging studies of large molecule, cellular/molecular, and cell/cell interactions. The success of the procedure hinges on the use of a reversible protecting group to modify reversibly &ohgr;-functionalized alkanethiols self-assembled on metal substrates.

Biomolecule and/or cellular arrays on metal surfaces and product produced thereby

Disclosed is a process to construct multi-component biomolecule or cellular arrays suitable for use in SPR imaging studies of large molecule, cellular/molecular, and cell/cell interactions. The success of the procedure hinges on the use of a reversible protecting group to modify reversibly .omega.-functionalized alkanethiols self-assembled on metal substrates.

Growing community of inventors

Big Flats, NY, United States of America

Anthony G Frutos