Twist Bioscience Corporation

Maxygen, Inc.

Codexis Mayflower Holdings, LLC

Anthony Cox

Jeremy S Minshull

Phillip A Patten

Linda A Castle

Siyuan Chen

Claes Gustafsson

Robin A Emig

Sridhar Govindarajan

Richard John Fox

S Christopher Davis

Rebecca Lynne Gorton

Daniel Siehl

Teddy Chen

Ajoy K Roy

Gjalt W Huisman

Willem P C Stemmer

Jon Edward Ness

Steven Henry Bass

Matthew B Tobin

Joseph A Affholter

Claus M Krebber

Venkiteswatan Mani Subramanian

Ying-Xin Zhang

Ling Yuan

Sebastian Treusch

Christopher S Davis

Stephen DelCardayre

Daniel L Siehl

Jeremy Minshull

Ajoy Apt120 Roy

Phil Patten

Rebecca Lynne Apt819 Gorton

Sridar Govindarajan

Disclosed herein are methods for the generation of highly accurate oligonucleic acid libraries encoding for predetermined variants of a nucleic acid sequence. The degree of variation may be complete, resulting in a saturated variant library, or less than complete, resulting in a selective library of variants. The variant oligonucleic acid libraries described herein may designed for further processing by transcription or translation. The variant oligonucleic acid libraries described herein may be designed to generate variant RNA, DNA and/or protein populations. Further provided herein are method for identifying variant species with increased or decreased activities, with applications in regulating biological functions and the design of therapeutics for treatment or reduction of disease.

Oligonucleic acid variant libraries and synthesis thereof

Disclosed herein are methods for the generation of nucleic acid libraries encoding for gRNA sequences. The gRNAs encoded by methods described herein may be single or double gRNA sequences. Methods described provide for the generation of gRNA libraries, as a DNA precursor or as a RNA transcription product, with improved accuracy and uniformity.

De novo synthesized nucleic acid libraries

Disclosed herein are methods for the generation of highly accurate nucleic acid libraries encoding for predetermined variants of a nucleic acid sequence. The nucleic acid sequence may encode for all or part of a TCR or a TCR-binding antigen. The degree of variation may be complete, resulting in a saturated variant library, or less than complete, resulting in a non-saturating library of variants. The variant nucleic acid libraries described herein may designed for further processing by transcription or translation. The variant nucleic acid libraries described herein may be designed to generate variant RNA, DNA and/or protein populations. Further provided herein are method for identifying variant species with increased or decreased activities, with applications in regulating biological functions and the design of therapeutics for treatment or reduction of a disease, such as cancer.

Variant libraries of the immunological synapse and synthesis thereof

Disclosed herein are methods for the generation of highly accurate nucleic acid libraries encoding for predetermined variants of a nucleic acid sequence. The nucleic acid sequence may encode for all or part of a reference domain of a CAR. The degree of variation may be complete, resulting in a saturated variant library, or less than complete, resulting in a non-saturating library of variants. The variant nucleic acid libraries described herein may be designed for further processing by transcription or translation. The variant nucleic acid libraries described herein may be designed to generate variant RNA, DNA and/or protein populations. Further provided herein are method for identifying variant species with increased or decreased activities, with applications in regulating biological functions and the design of therapeutics for treatment or reduction of a disease, such as cancer.

The present invention generally relates to methods of rapidly and efficiently searching biologically-related data space. More specifically, the invention includes methods of identifying bio-molecules with desired properties, or which are most suitable for acquiring such properties, from complex bio-molecule libraries or sets of such libraries. The invention also provides methods of modeling sequence-activity relationships. As many of the methods are computer-implemented, the invention additionally provides digital systems and software for performing these methods.

Methods, systems, and software for identifying functional bio-molecules

Methods, systems, and software for identifying functional biomolecules

The invention provides methods employing iterative cycles of recombination and selection/screening for evolution of whole cells and organisms toward acquisition of desired properties. Examples of such properties include enhanced recombinogenicity, genome copy number, and capacity for expression and/or secretion of proteins and secondary metabolites.

Evolution of whole cells and organisms by recursive sequence recombination

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Mountain View, CA, United States of America

Anthony Cox