Wake Forest University

Children's Medical Center Corporation

Massachusetts Institute of Technology

Other

North Carolina State University

The Administration of the Tulane Rducation Fund

Emory University

Children's Healthcare of Atlanta, Inc.

Anthony Atala

James J Yoo

Sang Jin Lee

Tao Xu

Shay Soker

Dennis Dice

Sean V Murphy

Weixin Zhao

Kyle W Binder

Aleksander Skardal

Samy Ashkar

Young Min Ju

In Kap Ko

Joel Stitzel

Grace Lim

Mark E Van Dyke

Charles Alfred Vacanti

George J Christ

Emmanuel C Opara

Justin M Saul

Keith T Paige

Steve J Hodges

Yuanyuan Zhang

Sittadjody Sivanandane

Hyun-Wook Kang

Mohammad Z Albanna

Benjamin S Harrison

Richard Czerw

James Mandell

Baisong Lu

Robert Samuel Langer

David J Mooney

Joseph P Vacanti

Linda G Griffith

Christopher B Doering

Richard A Wysk

H Trent Spencer

Linda Griffith-Cima

Colin Bishop

Hyunhee Ahn

Christopher A Sullivan

Gayoung Jeong

Paolo De Coppi

Young-Joon Seol

Paul Scarpinato

Benjamin C Harrison

Ivy Mead

David James Mccoul

Phillip Kim

Bryan Tillman

Qingguo Zhao

Joel D Stitzel, Jr

Robert T Wicks

Rohan A Shirwaiker

Caroline Elise Webster

Samuel Pendergraft

John D Jackson

Ola L A Harrysson

Goodwell Nzou

Hooman Sadri-Ardekani

Paul H Cohen

Yuan-Shin Lee

Peter M Prim

Elizabeth E Wicks

Molly F Purser

Katie L Basinger

Mitchell Ladd

Thomas Shupe

Anna Milanesi

Jennifer Huling

Gunter Schuch

W Terrance Godbey

Paolo Decoppi

Yoo James

Christopher D Porada

Maria Graca N D Almeida-Porada

Provided are compositions, systems, and methods useful for effecting gene editing in eukaryotic cells. Compositions include plasmids that encode one or more viral fusion proteins in which one or more viral proteins are fused with an aptamer-binding protein. Compositions also include plasmids that encode a non-viral nucleic acid sequence, wherein the non-viral nucleic acid sequence encodes a CRISPR system component. In some instances, the non-viral nucleic acid sequence also includes an aptamer sequence. The plasmids can be used to generate viral particles, including lentivirus-like particles that contain a viral fusion protein and a non-viral RNA sequence. Systems of producing such viral particles are provided. Also provided are methods of using the viral particles of the disclosure to effect gene editing in eukaryotic cells.

Lentiviral-based vectors and related systems and methods for eukaryotic gene editing

Provided herein are artificial lung organoids. The artificial lung organoids may include an epithelial cell layer comprising mammalian lung epithelial cells, a stromal cell layer comprising mammalian lung fibroblast cells and an endothelial cell layer comprising mammalian endothelial cells. The artificial lung organoids may optionally include a porous membrane between said epithelial cell layer and said stromal cell layer and/or between said stromal cell layer and said endothelial lung cell layer.

Multi-layer airway organoids and methods of making and using the same

Provided herein is a delivery system, including: (a) an optical sensor configured to detect data useful to create a map of a bodily surface; and (b) a printer operatively associated with the optical sensor and configured to deliver compositions (optionally including cells) to the bodily surface based upon the data or map. Methods of forming a tissue on a bodily surface of a patient in need thereof are also provided, as are methods, systems and computer program products useful for processing bodily surface data.

Delivery system

The present invention includes compositions and methods for wound healing and tissue regeneration. The compositions of the present invention comprise amniotic membrane powder. The compositions of the present invention comprise amniotic membrane powder and a scaffold. The methods of the present invention comprises applying a composition of the present invention to a subject to induce wound healing and tissue regeneration.

Amniotic membrane powder and its use in wound healing and tissue engineering constructs

The invention is directed to products and methods for preparing self-seeding vascular constructs generated as a bi-layered electrospun matrices, conjugated with EPC-specific antibodies and anti-thrombogenic agents on the inner surfaces of their lumens.

Vascular constructs

Provided are live, artificial, skin substitute products and methods of making and using the same, such as for wound treatment and compound testing, including compound testing for efficacy, toxicity, penetration, irritation and/or metabolism testing of drug candidates or compositions such as cosmetics.

Multi-layer skin substitute products and methods of making and using the same

A method of making an organ or tissue comprises: (a) providing a first dispenser containing a structural support polymer and a second dispenser containing a live cell-containing composition; (b) depositing a layer on said support from said first and second dispenser, said layer comprising a structural support polymer and said cell-containing composition; and then (c) iteratively repeating said depositing step a plurality of times to form a plurality of layers one on another, with separate and discrete regions in each of said layers comprising one or the other of said support polymer or said cell-containing composition, to thereby produce provide a composite three dimensional structure containing both structural support regions and cell-containing regions. Apparatus for carrying out the method and composite products produced by the method are also described.

Integrated organ and tissue printing methods, system and apparatus

The invention is directed to methods of inducing cell recruitment and tissue regeneration at a target site in a subject. It is also based, in part, on the discovery that a subject's own biologic resources and environmental conditions can be used for in situ tissue regeneration and thereby reduce or eliminate the need for donor cell procurement and ex vivo manipulation of such donor cells. Methods are disclosed for recruitment of a subject's own stem cells to a target region by inducing a sustained positive pressure at a target site, such as the kidney, thereby increasing the number of pluripotent cells capable of differentiating to regenerate the target tissue.

Directed stem cell recruitment

Provided herein is a method of forming a ventricular support device for a diseased heart, including providing imaging data of the diseased heart, forming a three-dimensional (3D) heart model based on the imaging data, providing strain data including a plurality of strain estimates for at least one segment of the diseased heart, mapping the plurality of strain estimates onto corresponding portions of the 3D heart model to form a 3D diseased heart model, based on the 3D diseased heart model, forming a model of the ventricular support device configured to surround at least a portion of the diseased heart and provide support based upon said strain estimates, and converting the model of the ventricular support device to a digital file useful for directing a 3D printer device to print said ventricular support device for said diseased heart. Also provided is a customized ventricular support device so produced.

Customized ventricular support device

Provided herein is a delivery system, including: (a) an optical sensor configured to detect data to create a map of a patient bodily surface; and (b) a dispenser operatively associated with the optical sensor and configured to deliver compositions (optionally including cells) to the patient bodily surface based upon the data or map. Methods of forming a tissue on a patient bodily surface of a patient in need thereof are also provided, as are methods, systems and computer program products useful for processing patient bodily surface data.

Provided herein are cell compositions useful for making artificial liver constructs. The cell composition my include, in combination, (a) hepatocyte cells, (b) Kuppfer cells, (c) hepatic stellate cells, (d) sinusoidal endothelial cells, and (e) cholangiocyte cells.

Methods of producing in vitro liver constructs and uses thereof

A method of treating hypoxic tissue such as wound tissue comprises contacting a composition to the hypoxic tissue in a hypoxia-treatment effective amount, the composition comprising a biodegradable polymer and an inorganic peroxide incorporated into the polymer.

Oxygen-generating compositions for enhancing cell and tissue survival in vivo

The present application relates to biomimetic three-dimensional (3D) scaffolds, constructs and methods of making the same. The three-dimensional scaffold can include a sacrificial internal cast and a durable external scaffold material, wherein the durable external scaffold material comprises a biocompatible material which completely surrounds the sacrificial internal cast and wherein the sacrificial internal cast be removed to yield a branching 3D network of hollow, vessel-like tubes that substantially mimics a native tissue or organ.

Vascular cast-based scaffolds and methods of making the same

Provided herein is an in vitro model of the blood brain barrier. In some embodiments, the model includes: an endothelial cell layer, and brain tissue layer comprising neuronal cells, and optionally one or more of astrocytes, pericytes, oligodendrocytes, and microglia. In some embodiments, the model further comprises a porous membrane between said endothelial cell layer and the neuronal cell layer. A microfluidic device comprising the same and methods of use thereof are also provided.

Blood brain barrier model and methods of making and using the same

Provided herein are stem cells and methods for producing a culture of stem cells from urine. The stem cells may be differentiated into an osteogenic, chondrogenic, adipogenic, endothelial, neurogenic or myogenic lineage. Methods of use of the cells are provided, including methods of treating a subject in need of a cell based therapy.

Stem cells from urine and methods for using the same

Growing community of inventors

Winston-Salem, NC, United States of America

Anthony Atala