Lifecell Corporation

The University of Texas System

Board of Regents

Anthony A Del Campo

Stephen A Livesey

Abhijit Nag

Carmen Piunno

Ken B Nichols

John G Linner

David P Ross

Christopher Coleman

Mark J Zaltzberg

Edward S Griffey

A method for processing and preserving an acellular collagen-based tissue matrix for transplantation is disclosed. The method includes the steps of processing biological tissues with a stabilizing solution to reduce procurement damage, treatment with a processing solution to remove cells, treatment with a cryoprotectant solution followed by freezing, drying, storage and rehydration under conditions that preclude functionally significant damage and reconstitution with viable cells.

Acellular dermal matrix and method of use thereof for grafting

This invention relates to methods, apparatus and solutions for cryopreserving microscopic biological materials for biologically extended periods of time. The method comprises treating a suspension of biological material, in an appropriate buffer, with a cryoprotectant or combination of cryoprotectants which raises the glass transition temperature range of the sample. One or more dry protectants may be added to the cryosolution. The cryosolution is then nebulized and rapidly cooled with novel apparatus, dried by molecular distillation, stored and then rehydrated in a buffer prior to its use. The solutions comprise novel mixtures of cryoprotectants.

Cryoprotective solutions comprising DMSO, PG, 2,3-butanediol,raffinose and PVP

Apparatus for cryopreparation, dry stabilization and rehydration of

Method of cryopreserving a suspension of biological material

Method for processing and preserving collagen-based tissues for

This invention relates to an apparatus and method for distillation drying of one or more biological samples. The drying apparatus includes a retaining assembly, a vacuum assembly, cooling means, monitoring means and control means for actively regulating the temperature and pressure conditions of biological tissue so that such tissue may be dried without substantial ultrastructural damage.

Method and apparatus for cryopreparing biological tissue

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Houston, TX, United States of America

Anthony A Del Campo