Teknologian Tutkimuskeskus Vtt Oy

Anssi Rantasalo

Joosu Kuivanen

Dominik Mojzita

Jussi Jäntti

Heiko Rischer

Kirsi-Marja Oksman-caldentey

Christopher Landowski

Jussi Jantti

A recombinant host cell is disclosed for producing psilocybin and related compounds, such as metabolic intermediates of the psilocybin biosynthesis. Also provided is a method of producing psilocybin and its synthesis intermediates and related compounds, such as metabolic intermediates of the psilocybin biosynthesis, in the host cell, as well as a production system for producing them.

Heterologous production of psilocybin

The present invention provides an expression system for a eukaryotic host, which comprises 1) an expression cassette comprising a core promoter, the core promoter controlling the expression of a DNA sequence encoding a synthetic transcription factor (sTF), and 2) one or more expression cassettes each comprising a DNA sequence encoding a desired product operably linked to a synthetic promoter, the synthetic promoter comprising a core promoter, and sTF-specific binding sites upstream of the core promoter. The present invention also provides a method for identifying universal core promoters for eukaryotic hosts, expression systems using universal core promoters, hosts comprising the systems, and methods for producing protein products in eukaryotic hosts.

Expression system for eukaryotic organisms

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Vtt, Finland

Anssi Rantasalo