Universitè Laval

Geneohm Sciences Canada, Inc.

Hema-quebec

Annie Roy

Christian Ménard

Steve Létourneau

Jessie Farah Fecteau

Patrick Boucher

Sonia Néron

Christian MÉNARD

Sonia Neron

A method for enrichment and isolation of microbial cells and microbial nucleic acids from a biological sample is described. The method comprises (i) adding to an initial volume of biological sample a differential cell lysis solution to obtain a final concentration of 0.1 to 1% of SDS in the sample; (ii) mixing the solution obtained in step (i) for a period of time sufficient to lyse the host cells present in the biological sample, while preserving the integrity of cells; and (iii) separating the microbial cells from the lysed host cells components. Differential cell lysis solutions and kits for practicing the method of the present invention are also provided.

Enrichment and isolation of microbial cells and microbial nucleic acids from a biological sample

This application relates to an in vitro method of producing a polyclonal IgG preparation. The method comprises (i) placing a polyclonal B-cell population enriched in IgG-secreting B cells in a culture medium; and (ii) culturing the polyclonal B-cell population under conditions enabling the production of the polyclonal IgG preparation from the polyclonal B-cell population. This improved method enables the production of antibodies (preferably IgG) and facilitates long-term culture of polyclonal B-cell populations.

Method for polyclonal immunoglobulin G production by human B cells

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Québec, Canada

Annie Roy