Monsanto Technology LLC

Ecogen, Inc.

Monsanto Company

Monsanto Technologies LLC

Annette C Slaney

William P Donovan

Mark J Rupar

Yuping Tan

Judith C Donovan

James Arthur Baum

Thomas M Malvar

Chih-Rei Chu

Elizabeth Pease

Timothy B Johnson

Willam P Donovan

Disclosed are novel insecticidal polypeptides, and compositions comprising these polypeptides, peptide fragments thereof, and antibodies specific therefor. Also disclosed are vectors, transformed host cells, and transgenic plants that contain nucleic acid segments that encode the disclosed &#x3b4;-endotoxin polypeptides. Also disclosed are methods of identifying related polypeptides and polynucleotides, methods of making and using transgenic cells comprising these polynucleotide sequences, as well as methods for controlling an insect population, such as Colorado potato beetle, southern corn rootworm and western corn rootworm, and for conferring to a plant resistance to a target insect species.

Coleopteran-toxic polypeptide compositions and insect-resistant transgenic plants

Disclosed is a novel &#x3b4;-endotoxin, designated CryET29, that exhibits insecticidal activity against siphonapteran insects, including larvae of the cat flea (), as well as against coleopteran insects, including the southern corn rootworm (), western corn rootworm (), Colorado potato beetle (), Japanese beetle (), and red flour beetle (). Also disclosed are nucleic acid segments encoding CryET29, recombinant vectors, host cells, and transgenic plants comprising a cryET29 DNA segment. Methods for making and using the disclosed protein and nucleic acid segments are disclosed as well as assays and diagnostic kits for detecting cryET29 and CryET29 sequences in vivo and in vitro.

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Disclosed arestrains comprising novel crystal proteins which exhibit insecticidal activity against coleopteran insects including red flour beetle larvae () and Japanese beetle larvae (). Also disclosed are novelcrystal toxin genes, designated cryET33 and cryET34, which encode the colepteran-toxic crystal proteins, CryET33 (29-kDa) crystal protein, and the cryET34 gene encodes the 14-kDa CryET34 crystal protein. The CryET33 and CryET34 crystal proteins are toxic to red flour beetle larvae and Japanese beetle larvae. Also disclosed are methods of making and using transgenic cells comprising the novel nucleic acid sequences of the invention.

Polynucleotide encoding coleopteran-toxic polypeptide

Disclosed are transgenic plants, seeds, and progeny from such plants comprising novel nucleic acid sequences encoding both CryET33 and CryET34 crystal proteins. The novel nucleic acid sequences comprise SEQ ID NO:11. CryET33 and CryET34 crystal proteins comprise SEQ ID NO:3 and SEQ ID NO:4 and exhibit insecticidal activity against coleopteran insects including red flour beetle larvae () and Japanese beetle larvae ().

Insect-resistant transgenic plants transformed with CryET33 and CryET34-encoding nucleic acids

Coleopteran-toxic polypeptide compositions

Disclosed are transgenic plants, seeds, and progeny from such plants comprising a cryET29 DNA segment. The cryET29 DNA segment encodes CryET29 crystal protein which comprises SEQ ID NO:2 and exhibits insecticidal activity against siphonapteran insects, including larvae of the cat flea (), as well as against coleopteran insects, including the southern corn rootworm (), western corn rootworm (), Colorado potato beetle (), Japanese beetle (), and red flour beetle ().

Plants transformed with CryET29-encoding nucleic acids

Disclosed are nucleic acids encoding novel insecticidal &#x3b4;-endotoxin polypeptides, and vectors, transformed host cells, and transgenic plants that contain the nucleic acids. Also disclosed are methods of making and using transgenic cells comprising these polynucleotide sequences, as well as methods for controlling an insect population, such as Colorado potato beetle, southern corn rootworm and western corn rootworm, and for conferring to a plant resistance to a target insect species.

Nucleic acids encoding coleopteran-toxic polypeptides and insect-resistant transgenic plants comprising them

Bacillus thuringiensis cryET33 and cryET34 compositions and uses therefor

Disclosed is a novel &dgr;-endotoxin, designated CryET29, that exhibits insecticidal activity against siphonapteran insects, including larvae of the cat flea ( ), as well as against coleopteran insects, including the southern corn rootworm ( ), western corn rootworm ( ), Colorado potato beetle ( ), Japanese beetle ( ), and red flour beetle ( ). Also disclosed are nucleic acid segments encoding CryET29, recombinant vectors, host cells, and transgenic plants comprising a cryET29 DNA segment. Methods for making and using the disclosed protein and nucleic acid segments are disclosed as well as assays and diagnostic kits for detecting cryET29 and CryET29 sequences in vivo and in vitro.

Bacillus thuringiensis CryET29 compositions toxic to coleopteran insects and ctenocephalides SPP

Disclosed are novel insecticidal polypeptides, and compositions comprising these polypeptides, peptide fragments thereof, and antibodies specific therefor. Also disclosed are vectors, transformed host cells, and transgenic plants that contain nucleic acid segments that encode the disclosed &dgr;-endotoxin polypeptides. Also disclosed are methods of identifying related polypeptides and polynucleotides, methods of making and using transgenic cells comprising these polynucleotide sequences, as well as methods for controlling an insect population, such as Colorado potato beetle, southern corn rootworm and western corn rootworm, and for conferring to a plant resistance to a target insect species.

Bacillus thuringiensis CryET29 compositions toxic to coleopteran insects and Ctenocephalides SPP

Disclosed are strains comprising novel crystal proteins which exhibit insecticidal activity against coleopteran insects including red flour beetle larvae ( ) and Japanese beetle larvae ( ). Also disclosed are novel crystal toxin genes, designated cryET33 and cryET34, which encode the colepteran-toxic crystal proteins, CryET33 (29-kDa) crystal protein, and the cryET34 gene encodes the 14-kDa CryET34 crystal protein. The CryET33 and CryET34 crystal proteins are toxic to red flour beetle larvae and Japanese beetle larvae. Also disclosed are methods of making and using transgenic cells comprising the novel nucleic acid sequences of the invention.

Bacillus thuringiensis cryet33 and cryet34 compositions and uses thereof

Disclosed are,strains comprising novel crystal proteins which exhibit insecticidal activity against coleopteran insects including red flour beetle larvae (,) and Japanese beetle larvae (,). Also disclosed are novel,crystal toxin genes, designated cryET33 and cryET34, which encode respectively the coleopteran-toxic proteins, CryET33 (29-kDa) crystal protein, and CryET34 (14-kDa) crystal protein. Also disclosed are methods of making and using transgenic cells comprising the novel nucleic acid sequences of the invention.

Bacillus thuringiensis CryET33 and CryET34 compositions and uses therefor

Disclosed are,strains comprising novel crystal proteins which exhibit insecticidal activity against coleopteran insects including red flour beetle larvae (,) and Japanese beetle larvae (,). Also disclosed are novel,crystal toxin genes, designated cryET33 and cryET34, which encode the colepteran-toxic crystal proteins, CryET33 (29-kDa) crystal protein, and the cryET34 gene encodes the 14-kDa CryET34 crystal protein. The CryET33 and CryET34 crystal proteins are toxic to red flour beetle larvae and Japanese beetle larvae. Also disclosed are methods of making and using transgenic cells comprising the novel nucleic acid sequences of the invention.

Growing community of inventors

Burlington, NJ, United States of America

Annette C Slaney