Gist-brocades N.v.

Dsm N.v.

Annemarie Eveline Veenstra

Willem Van Hartingsveldt

Petrus Andreas Van Paridon

Pieter Van Solingen

Bertus Pieter Koekman

Juan F Martin

Rudolf Gm Luiten

Jose Luis Barredo

Robert Fm Van Gorcom

Henk Van Liempt

Gerardus Cornelius Selten

Lucia Helena Van Der Voort

Harriet Palissa

Gerardus C M Selten

Rudolf G M Luiten

Robert F M Van Gorcom

Martinus A Groenen

Christina Esmahan

Santiago Gutierez

Martien A Groenen

Bruno Diez Garcia

Hans Von Doehren

Eduardo Montenegro Prieto

Gerardus Cm Selten

Santiago Gutierrez

Bruno Diez

Emilio Alvarez

Martien Am Groenen

Robert F Van Gorcom

Petrus A Van Paridon

Rudolf G Luiten

Gerardus C Selten

A nucleotide sequence encoding phytase has been isolated and cloned. The coding sequence has been inserted into an expression construct which in turn has been inserted into a vector capable of transforming a microbial expression host. The transformed microbial hosts may be used to economically produce phytase on an industrial scale. The phytase produced via the present invention may be used in a variety of processes requiring the conversion of phytate to inositol and inorganic phosphate.

Cloning and expression of phytase from aspergillus

Novel methods and compositions are provided for the enhanced production of .beta.-lactam antibiotics. The process is exemplified by the production of penicillin. In addition, the P. chrysogenum and A. chrysogenum .delta.-(L-.alpha.-aminoadipyl)-L-cysteinyl-D-valine synthetase genes have been isolated and sequenced. Also methods are provided for the production of .delta.-(L-.alpha.-aminoadipyl)-L-cysteinyl-D-valine synthetase using recombined DNA techniques.

Method for influencing .beta.-lactam antibiotic production and for

Cloning and expression of microbial phytase

Clustered antibiotic biosynthetic genes are employed for improvement of production of the antibiotic in microorganisms and for the isolation of other genes involved in the biosynthesis of the antibiotic. The invention is exemplified with improved production of penicillin in Penicillium chrysogenum, with the isolation of another clustered biosynthetic gene(s) and with the expression of penicillin biosynthetic genes in Acremonium chrysogenum.

Method and compositions for enhancing production of secondary

Novel methods and compositions are provided for the enhanced production of secondary metabolites. Methods are provided for identifying sequences which when transformed into secondary metabolite producing hosts, enhance the production of secondary metabolite. The process is exemplified for penicillin. In addition, the P. chrysogenum acyltransferase gene is isolated and sequenced.

Method for identifying and using biosynthetic genes for enhanced

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Nieuw Vennep, Netherlands

Annemarie Eveline Veenstra