Northwestern University

Wisconsin Alumni Research Foundation

Chiron Corporation

Other

Leland Stanford Junior University

University of California

University of Ottawa

Annelise E Barron

Ronald N Zuckermann

Michelle Dohm

Samuel Helmer Gellman

Shannon S Stahl

Robert Meagher

Cindy W Wu

Brendan P Mowery

Phillip Byron Messersmith

Andrea R Statz

Cheuk-Wai Kan

Thomas N Chiesl

Erin Doherty

Thomas J Meade

Frank W Mercer

Cheuk Wai Kan

Achim Karger

Scott Mack

Wyatt N Vreeland

Jen-Kuei Liu

Gary W Slater

Bharti Solanki

Cevat Akin

Ann M Czyzewski

Hans Michael Wenz

Laurette C McCormick

Vinod Srinivasan

Brett A Buchholz

Kavi P Mehta

Jacob M Zahn

Nicolynn E Davis

Nathaniel Chongslriwatana

Wei Huang

Tyler M Miller

James A Patch

Jennifer Sue Lin

Nathaniel P Chongsiriwatana

Jennifer Coyne Albrecht

Lindsay K Sulzer

Xiaoxiao Wang

Steven R Bull

Christopher P Fredlake

Andrea Statz

Provided herein is an electrophoresis separation medium comprising: (a) a non-crosslinked or sparsely cross-linked polymer or copolymer; (b) one or more denaturant compounds, in an amount sufficient to inhibit re-naturation of single stranded polynucleotides; (c) an aqueous solvent; (d) optionally, a wall-coating material suited to inhibition of electroosmotic flow; and (e) optionally, an organic water miscible solvent such as DMSO or acetonitrile, wherein the electrophoresis separation medium exhibits functional stability for at least seven days at 23&#xb0; C. Also provided herein are sieving compositions, including polymer-based sieving compositions, for molecular sieving as well as related kits, devices and methods of use. Such compositions can be useful for separation of biomolecules such as nucleic acids, proteins, glycoproteins and glycans.

Compositions, methods, kits and devices for molecular analysis

Non-natural oligomers have recently shown promise as functional analogues of lung surfactant proteins B and C (SP-B and SP-C), two helical and amphiphilic proteins that are critical for normal respiration. The generation of non-natural mimics of SP-B and SP-C has previously been restricted to step-by-step, sequence-specific synthesis, which results in discrete oligomers that are intended to manifest specific structural attributes. Presented herein an alternative approach to SP-B mimicry that is based on sequence-random copolymers containing cationic and lipophilic subunits. These materials, members of the nylon-3 family, are prepared by ring-opening polymerization of &#x3b2;-lactams. The best of the nylon-3 polymers display promising in vitro surfactant activities in a mixed lipid film. Pulsating bubble surfactometry data indicate that films containing the most surface-active polymers attain adsorptive and dynamic-cycling properties that surpass those of discrete peptides intended to mimic SP-B. Attachment of an N-terminal octadecanoyl unit to the nylon-3 copolymers affords further improvements by reducing the percent surface area compression to reach low minimum surface tension.

Nylon-3 co-polymers and synthetic lung surfactant compositions containing same

Immobilizable antimicrobial compounds incorporating antimicrobial and/or antifouling components, as can be adhered to various device structures and components.

Surface-immobilized antimicrobial peptoids

Non-natural oligomers have recently shown promise as functional analogs of lung surfactant proteins B and C (SP-B and SP-C), two helical and amphiphilic proteins that are critical for normal respiration. The generation of non-natural mimics of SP-B and SP-C has previously been restricted to step-by-step, sequence-specific synthesis, which results in discrete oligomers that are intended to manifest specific structural attributes. Presented herein an alternative approach to SP-B mimicry that is based on sequence-random copolymers containing cationic and lipophilic subunits. These materials, members of the nylon-3 family, are prepared by ring-opening polymerization of &#x3b2;-lactams. The best of the nylon-3 polymers display promising in vitro surfactant activities in a mixed lipid film. Pulsating bubble surfactometry data indicate that films containing the most surface-active polymers attain adsorptive and dynamic-cycling properties that surpass those of discrete peptides intended to mimic SP-B. Attachment of an N-terminal octadecanoyl unit to the nylon-3 copolymers affords further improvements by reducing the percent surface area compression to reach low minimum surface tension.

The present disclosure provides substantially monodisperse random coil polypeptides, vectors encoding the polypeptides, conjugates containing the polypeptides, methods for their preparation, and their uses in nucleic acid separations, DNA sequencing, and other applications requiring high monodispersity.

Monodisperse random coil proteins and bioconjugates thereof

Non-natural oligomers have recently shown promise as functional analogues of lung surfactant proteins Band C (SP-B and SP-C), two helical and amphiphilic proteins that are clitical for normal respiration. The generation of non-natural mimics of SP-B and SP-C has previously been restlicted to step-by-step, sequence-specific synthesis, which results in discrete oligomers that are intended to manifest specific structural attributes. Presented herein an alternative approach to SP-R mimicry that is based on sequence-random copolymers containing cationic and lipophilic subunits. These materials, members of the nylon-3 family, arc prepared by ling-opening polymelization of 13-lactams. The best of the nylon-3 polymers display promising in vitro surfactant activities in a mixed lipid film. Pulsating bubble surfactometry data indicate that films containing the most surface-active polymers attain adsorptive and dynamic-cycling properties that surpass those of discrete peptides intended to mimic SP-B.

Embodiments of the present disclosure provide for hydrogels, methods of making hydrogels, methods of using hydrogels, methods of isolating, trapping, attracting, and/or killing cancer cells, and the like.

Hydrogels, methods of making hydrogels, methods of using hydrogels, and methods of isolating, trapping, attracting, and/or killing cancer cells

The present invention provides spreading agents based on sequence-specific oligomers comprising a peptoid, a peptide-peptoid chimera, a retropeptoid or a retro(peptoid-peptide) chimera, and methods for using the same, including for the treatment of respiratory distress of the lungs. The spreading agents are sequence-specific oligomers, including retrosequence-specific oligomers, based on a peptide backbone, that are designed as analogs of surfactant protein-B or surfactant protein-C.

Polypeptoid pulmonary surfactants

Antimicrobial peptoid compounds and related compositions as can be used against bacteria effectively and selectively.

Selective poly-N-substituted glycine antibiotics

Polymeric compounds and related methods and apparatus, as can be used in a wide range of RNA and DNA separations.

Thermo-gelling matrices and related methods for microchannel DNA sequencing

Linear acrylamide copolymer compounds which can comprise monomeric components comprising at least one N-substituted moiety capable of physical cross-linking, and related compositions and methods of use.

Physically crosslinked copolymer compounds and related compositions and methods for electrophoretic separation

The present invention provides compositions and methods for performing free-solution conjugate analysis of nucleic acid molecules. For example, the present invention provides multiplexed single-base extension assays for genotyping. In particular, the present invention provides a series of disperse polyamide 'drag tags' for use in achieving high-resolution separation of nucleic acid reaction products.

Compositions and methods for free-solution conjugate nucleic acid analysis

The present application provides for compositions and methods of creating and applying contrast agents that find use in magnetic resonance imaging (MRI). In particular, contrast agents that comprise monodisperse, protein polymer backbones capable of chelating multiple paramagnetic ions resulting in high relaxivity for use in enhancing MRI signal intensity.

Compositions and methods for magnetic resonance imaging contrast agents

Growing community of inventors

Evanston, IL, United States of America

Annelise E Barron