Miltenyi Biotec B.v. Co., Kg

Miltenyi Biotec, Gmbh

Multenyi Biotec, Gmbh

Anne Richter

Stefan Miltenyi

Mario Assenmacher

Andrew Kaiser

Michael Lutteropp

Michaela Niemöller

Christian Dose

Volker Nolle

Jonathan Fauerbach

Tina Borke

Volker Nölle

This disclosure provides a system for preventing or reducing side effects in a patent undergoing immunotherapy to remove diseased cells that express a target antigen: for example, by CAR T cell therapy. Side effects can ensue from concurrent depletion of hematopoietic cells bearing the same target antigen. A population of engineered hematopoietic cells is prepared by obtaining healthy hematopoietic cells from the patient or a third party donor, and using them to produce engineered hematopoietic cells. The engineered cells either do not express the target antigen, express it at a lower density, or express it in a modified form. The engineered hematopoietic cells are formulated for administration to the patient, whereupon they reconstitute hematopoietic cell function, thereby preventing or reducing the side effects.

Reducing side effects of immunotherapy using genetically modified hematopoietic cells

The invention provides a system that comprises pharmaceutical agents for use in immunotherapy for reducing the side-effects of an antigen-recognizing receptor against antigen-expressing non-target cells in an individual. The system includes an antigen-recognizing receptor that specifically recognizes an antigen on target cells and at least on one hematopoietic cell type in the individual. The antigen-recognizing receptor is exemplified by chimeric antigen receptors (CAR) be expressed on the surface of an immune effector cells. The system also includes hematopoietic cells resistant to recognition of the same antigen by the antigen-recognizing receptor.

Immunotherapy of cancer patients using CAR T or CAR NK cells combined with hematopoietic cells that have a modified or deleted target antigen

The invention is directed to a method for preparing a detection reagent for CAR expressing cells according to general formula (I)(X-S)-D  (I)

Reagents for detection of chimeric antigen receptor cells

Combination of immune effector cells specific for a target antigen and hematopoietic calls that express the target antigen in an altered form

Immunotherapy method comprising administering a combination of immune effector cells and non-target hematopoietic cells

Combination of immune effector cells specific for a target antigen and hematopoietic cells that express the target antigen in an altered form

The invention is directed to a detection reagent for CAR expressing cells according to general formula (1)X&#x2014;S&#x2014;D The detection agent can be used in a method to detect cells having an antigen binding domain of a chimeric antigen receptor (CAR) binding the detection reagent and preferable to remove the detection moiety D from the detected cell.

Assay for detection of chimeric antigen receptor T cells

Combination immunotherapy of antigen-recognizing receptors and hematopoietic cells for the treatment of diseases

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Bergisch Gladbach, Germany

Anne Richter