Polyplus-Transfection Sa

Polyplus Transfection

Anne-Laure Bolcato Bellemin

Jean-Paul Behr

Patrick Erbacher

Patrick Neuberg

The invention relates to compositions comprising double-stranded oligonucleotides of identical or different sequences and/or length, said oligonucleotides having sequencesNN. . . NN. . . Nwherein&#x2014;N. . . Nis half of a double-stranded 19-28 mer oligonucleotide of sequence complementary to a target nucleic acid sequence present in a living cell, and&#x2014;N. . . Nis a 3-50 mer overhang of sequence allowing oligomerization of said double-stranded oligonucleotide. Compositions of transfection comprising said oligonucleotide compositions and there used for therapeutical application.

Oligonucleotides for RNA interference and biological applications thereof

The invention relates to compositions comprising double-stranded oligonucleotides of identical or different sequences and/or length, said oligonucleotides having sequencesNN. . . NN. . . Nwherein&#x2014;N. . . Nis half of a double-stranded 19-28mer oligonucleotide of sequence complementary to a target nucleic acid sequence present in a living cell, and&#x2014;N. . . Nis a 3-50mer overhang of sequence allowing oligomerization of said double-stranded oligonucleotide. Compositions of transfection comprising said oligonucleotide compositions and there used for therapeutical application.

The invention relates to compositions of transfection comprising an oligonucleotide and an amphiphilic cationic molecule of formula (I) wherein, &#x2014;X is N&#x2014;R, S or O, Rbeing a C1-C4 alkyl radical or an hydroxylated C3-C6 alkyl radical, Rand R, identical or different, represent H or a C1-C4 alkyl radical, or Rand Rare linked together to form a saturated or unsaturated cycle or a heterocycle having 5 or 6 elements, E is a C1-C5 alkyl spacer, Rand R, identical or different, represent saturated or unsaturated, linear or branched, C10-C36 hydrocarbon or fluorocarbon chains, optionally comprising C3-C6 cycloalkyl, A&#x2212; is a biocompatible anion. The invention relates to compositions active for oligonucleotides delivery into eukaryotic cells in culture, ex vivo or in vivo. The invention relates to compositions of transfection comprising an oligonucleotide active for RNA interference. Such compositions can be used as tools for biological studies or as drugs for therapies.

Growing community of inventors

Strasbourg, France

Anne-Laure Bolcato Bellemin