Lallemand Hungary Liquidity Management LLC

Stellenbosch University

Mascoma Corporation

Danstar Ferment Ag

Universiteit Stellenbosch

Anne K Warner

John E McBride

Chhayal V Gandhi

Elena E Brevnova

Erin Wiswall

Mark Mellon

Trisha Barrett

Nicky Caiazza

Aaron Argyros

Vineet Rajgarhia

Merja Penttilä

Charles F Rice

Allan C Froehlich

Ryan Skinner

Riaan Den Haan

Emily Stonehouse

Shital A Tripathi

Haowen Xu

Kristen M Deleault

John S Bardsley

Indraneel Shikhare

Kevin S Wenger

Frank Agbogbo

Yin-Ying Chiu

Jennifer Gosselin

Vineet B Rajgarhia

Alan Belcher

Abigail Foster

Heidi H Hau

Alan Benjamin Gilbert

D Aaron Argyros

Deidre Willes

Naomi Thorngren

Matti Siika-Aho

Marja Ilmen

Emile Van Zyl

Jaana Uusitalo

Danie Lagrange

Jim Flatt

Heidi Lau

Elena Brevnova

Merja Penttila

Trisha F Barrett

Abigail S Foster

Chhayal Ghandi

Jeffrey D Keating

Emily A Stonehouse

Heidi Hanson Hau

Dan Murphy

Alan Froehlich

Jeff Villari

Shaunita Rose

John E Mcbride

The present invention is directed to a yeast strain, or strains, secreting a full suite, or any subset of that full suite, of enzymes to hydrolyze corn starch, corn fiber, lignocellulose, (including enzymes that hydrolyze linkages in cellulose, hemicellulose, and between lignin and carbohydrates) and to utilize pentose sugars (xylose and arabinose). The invention is also directed to the set of proteins that are well expressed in yeast for each category of enzymatic activity. The resulting strain, or strains can be used to hydrolyze starch and cellulose simultaneously. The resulting strain, or strains can be also metabolically engineered to produce less glycerol and uptake acetate. The resulting strain, or strains can also be used to produce ethanol from granular starch without liquefaction. The resulting strain, or strains, can be further used to reduce the amount of external enzyme needed to hydrolyze a biomass feedstock during an Simultaneous Saccharification and Fermentation (SSF) process, or to increase the yield of ethanol during SSF at current saccharolytic enzyme loadings. In addition, multiple enzymes of the present invention can be co-expressed in cells of the invention to provide synergistic digestive action on biomass feedstock. In some aspects, host cells expressing different heterologous saccharolytic enzymes can also be co-cultured together and used to produce ethanol from biomass feedstock.

Yeast expressing saccharolytic enzymes for consolidated bioprocessing using starch and cellulose

The present invention provides for heterologous expression of termite and termite-associated symbiont cellulases. The cellulases can, for example, be codon-optimized and expressed in yeast host cells, such as the yeast. The cellulases can also be co-expressed in host cells with other cellulases. The expression in such host cells of the termite and termite-associated symbiont cellulases, and variants and combinations thereof, result in yeast with improved cellulosic activity. Thus, such genes and expression systems are useful for efficient and cost-effective consolidated bioprocessing systems.

Heterologous expression of termite cellulases in yeast

The present invention provides a microorganism capable of fermenting arabinose to a desired product such as ethanol. In some embodiments, the organism is also capable of fermenting xylose. In some embodiments, the organism is capable of fermenting arabinose and xylose, and expresses one or more cellulases.

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Heterologous expression of termite cellulases yeast

The present invention provides for the isolation and characterization of the cbh1 gene from. In particular, the present invention provides for the nucleic acid and amino acid sequences ofcbh1, and domains, variants and derivatives thereof. The present invention further provides for the heterologous expression ofCbh1 in host cells, including yeast, e.g.,. Expression ofCbh1 in host cells will augment cellulose digestion and facilitate ethanol production by those host cells on cellulosic substrates. In certain embodiments, heterologous expression inis in coordination with heterologous expression of other known, or newly identified saccharolytic enzymes. Therefore, the present invention also provides that the novelCbh1 gene can utilized in a consolidated bioprocessing system.

Growing community of inventors

Lebanon, NH, United States of America

Anne K Warner