Somagenics, Inc.

Realseq Biosciences, Inc.

University of California

Integrated Dna Technologies, Inc.

Anne Dallas

Brian H Johnston

Heini Ilves

Sergei A Kazakov

Qing Ge

Mark Aaron Behlke

Sumedha Jayasena

Sergio Barberan-Soler

Kathy Lieberman

Catharina Casper-Lindley

Harry F Noller

Jamie H D Cate

Marat M Yusupov

Gulnara Zh Yusupova

Albion E Baucom

Laura Lancaster

Disclosed herein are methods and compositions for the detection of small RNAs in a sample. The methods and compositions disclosed herein may be used for preparing sequencing libraries of the small RNAs, fragments of RNAs and DNAs.

Methods of library construction for polynucleotide sequencing

Wound healing is a complex homeostatic process in which several distinct types coordinate to repair a physical damage. Failure to close wounds contributes to the pathology of conditions like diabetes mellitus, particularly in the elderly. Presented herein are molecules, pharmaceutical compositions, and methods for applying small RNA oligonucleotide technology to wound healing. Small RNA oligonucleotide approaches as disclosed herein provide a therapeutic strategy for improving both basal and pathological wound healing.

Methods and compositions of short small hairpin RNAs and microRNAs for wound healing

Aspects of the present invention include the production and use of chemically modified RNAi agents (e.g., shRNAs) in gene silencing applications. The chemically modified RNAi agents disclosed herein have reduced immunostimulatory activity, increased serum stability, or both, as compared to a corresponding RNAi agent not having the chemical modification. Compositions containing chemically modified RNAi agents according to aspects of the present invention (including pharmaceutical compositions) and kits containing the same are also provided.

Chemical modification of short small hairpin RNAs for inhibition of gene expression

Methods and compositions of short small hairpin RNAS and microRNAS for wound healing

Disclosed herein are compositions and methods for the processing, amplification and detection of polynucleotides using target-specific oligonucleotides (TSOs). Hybridization of TSOs to target polynucleotides guides target processing into and purification of small target fragments that then can be amplified and detected with high sensitivity and reproducibility. The method is specifically beneficial for highly degraded polynucleotides found in biological samples.

Methods and compositions for detecting polynucleotides and fragments thereof

Methods, compositions, and kits that include small hairpin RNA (shRNA) useful for inhibition of gene expression, such as viral-mediated gene expression, are described.

Short hairpin RNAs for inhibition of gene expression

5.5 Angstrom resolution x-ray crystallographic structures of 70S ribosome complexes containing messenger RNA and tranfer RNA (tRNA), or tRNA analogs, are provided. The resolution has been enhanced by fitting atomic resolution structures of 30S and 50S subunits onto the 5.5 anstrong electron density map. The enhanced structure reveals regions of structural differences between the 70S complex and the structures of the individual 30S and 50S components. Pharmacophore design to discover novel inhibitors or activators may be carried out using the enhanced 5.5 Angstrom 70S structure.

X-ray crystal structures of functional ribosome complexes containing transfer RNA and model messenger RNAs and methods of use

Growing community of inventors

Santa Cruz, CA, United States of America

Anne Dallas