E.i. Dupont De Nemours and Company

Danisco US Inc.

Annapurna Sachan

Richard Ray Bott

Qiong Cheng

Pierre E Rouviere

Sina Pricelius

Kristin Ruebling-Jass

Qi Chen

Neeraj Pandey

Ritu Bhalla

Stepan Shipovskov

Ajit Kumar Satapathy

Disclosed herein are cellulase variants, or active fragments thereof, and polynucleotides encoding same, where the cellulase variants, or active fragments thereof, have endoglucanase activity. Also disclosed herein are compositions comprising the cellulase variants, or active fragments thereof; vectors and/or host cells comprising the polynucleotides encoding the cellulase variants, or active fragments thereof; and methods for making and/or using the cellulase variants, or active fragments thereof and/or compositions containing same; where the cellulase variants, or active fragments thereof, have endoglucanase activity.

Polypeptides with endoglucanase activity and uses thereof

Methods are provided to obtain recombinant microbial cells having at least one genetic modification that increase the buoyant density of a recombinant microbial cell or the buoyant density of inclusion bodies produced within a recombinant microbial cell. Exemplified are genetic modifications that increase the buoyant density of a recombinant microbial cell expressing heterologous peptides and polypeptides. Increasing expression of the genes ysaB, glyQ, glyS or a combination thereof within the recombinant microbial cell produces cells or inclusion bodies having higher buoyant density. A similar effect was achieved by decreasing or disrupting expression of the endogenous gltA gene. Increases in buoyant density render peptide production more efficient with respect to time and costs.

Recombinant host cells having an increase in buoyant density

Growing community of inventors

Kanpur, India

Annapurna Sachan