Dharmacon, Inc.

Elitech Holding B.v.

Elitechgroup B.v.

Ge Healthcare Dharmacon, Inc.

Annaleen Vermeulen

Anastasia Khvorova

Devin Leake

Barbara Robertson

Nicolaas M J Vermeulen

Jon Michael Karpilow

Walt Mahoney

Christina Yamada

Rob Kaiser

Joshua Stahl

Brady Culver

Anja Smith

Abel Licon

Scott Baskerville

Yuriy Fedorov

Joshua A Stahl

Nicolaas Vermeulen

The present invention is directed to collections of templates for molecules such as RNA, as well as templates, devices, kits and methods for generating molecules from these collections. Through the use of various embodiments of the present invention one may efficiently and effectively obtain selected RNA molecules such as siRNA, shRNA, miRNA mimics and inhibitors, lncRNA, antisense RNA, aptamers, ribozymes, and sgRNA and sets of those molecules.

Templates, libraries, kits and methods for generating molecules

Compositions and methods for inhibiting the actions of non-coding RNAs such as miRNAs and piRNAs are provided. The compositions comprise single or double stranded oligonucleotides conjugated with Minor Groove Binders ('MGBs'). The oligonucleotides can vary in length, can contain nucleotides having one or more modifications, and have regions that are substantially complementary to one or more mature miRNAs or piRNAs.

Minor groove binder (MGB)-oligonucleotide miRNA antagonists

Disclosed are methods of enhancing functionality of duplex oligonucleotides and compositions made by the methods. The duplex oligonucleotides include siRNAs, miRNA mimics, and piRNA mimics which contain modified nucleotides and mismatches between the two strands of the molecule at specific nucleotide positions.

Duplex oligonucleotides with enhanced functionality in gene regulation

The disclosure provides methods for inhibiting the activity of a miRNA cluster in a cell, and also for screening a cell for a phenotype(s) of interest resulting from inhibition of a miRNA cluster. The methods use a cluster pool which comprises at least one miRNA inhibitor specific for each miRNA in the miRNA cluster. MiRNA inhibitors are described that induce apoptosis in breast cancer cells and hence are useful in the treatment of breast cancer. The disclosure also provides pharmaceutical compositions which are useful for the treatment of breast cancer; methods for inducing the nuclear translocation of NF-&#x3ba;B in a breast cancer cell; methods for inducing the nuclear translocation of c-Jun in a breast cancer cell; method for inhibiting the nuclear translocation of NF-&#x3ba;B in a breast cancer cell; and methods for providing prognostic medical information relating to breast cancer progression.

Screening of micro-RNA cluster inhibitor pools

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Lafayette, CO, United States of America

Annaleen Vermeulen