Harvard College

President and Fellows of Havard College

Anna Laromaine Sagué

George M Whitesides

Charles R Mace

Ratmir Derda

Jabulani Barber

Rebecca Cademartiri

Three-dimensional cellular arrays, methods of making three-dimensional cellular arrays, and methods of identifying agents using the arrays are disclosed.

Paper-based cellular arrays

Alginate hydrogel fibers and related materials as well as methods for preparing such materials are provided. An alginate hydrogel fiber includes water in an amount of more than about 92% by weight of the fiber and a cross-linked alginate in an amount of about 0.1% to about 8% by weight of the fiber, wherein the cross-link is a cation. An alginate hydrogel paper includes one or more alginate hydrogel fibers, which form a non-woven matrix. Three-dimensional cellular arrays are also provided, wherein the alginate hydrogel making up the alginate paper is substantially index-matched with a predetermined culture medium. A method for making alginate hydrogel fiber and a method for index-matching alginate hydrogel paper with culture medium are provided. A kit for conducting biochemical, diagnostic, cellular, and/or non-cellular analysis comprises alginate hydrogel paper index-matched to culture medium.

Alginate hydrogel fibers and related materials

Growing community of inventors

Cambridge, MA, United States of America

Anna Laromaine Sagué