Symbiocelltech, LLC

Anna L Gooch

Christof Westenfelder

Ping Zhang

Zhuma Hu

Disclosed herein is a method of generating exosomes (extracellular nanovesicles) and/or neo-islets from mesenchymal or adipose stem cells or islets, or other cells in a Hollow-Fiber-based Cell Expansion (HFCE) System. Such exosomes and/or neo-islets may be used for the treatment of T1DM, T2DM, or associated microvascular disease.

Adaptation of hollow-fiber-based cell culture technology for the manufacturing of (1) neo-islets, employed for the treatment of type 1 and type 2 diabetes mellitus, and (2) the generation of exosomes from various cell types, used in the treatment of different organ injuries and diseases

Described are Neo-Islets comprising: a) dedifferentiated islet cells and mesenchymal and/or adipose stem cells; or b) redifferentiated islet cells and mesenchymal and/or adipose stem cells where the cells have been treated so as to facilitate redifferentiation. Further described herein are methods of generating Neo-Islets, the methods comprising: culturing a) dedifferentiated islet cells and mesenchymal and/or adipose stem cells; or b) redifferentiated islet cells and mesenchymal and/or adipose stem cells; on a surface that promotes the formation of cell clusters. Also described are methods of treating a subject, the methods comprising: providing to the subject Neo-Islets described herein. Additionally described are methods of treating a subject suffering from Type 1 Diabetes Mellitus, Type 2 Diabetes Mellitus, and other types of insulin-dependent diabetes mellitus, or impaired glucose tolerance by providing to the subject Neo-Islet as described herein. Additionally described are methods of treatment in which intraperitoneal administration of islet-sized Neo-Islets composed of high numbers of mesenchymal stem cells and cultured islet cells, durably and reversibly treats, without hypoglycemia, both streptozotocin-induced and spontaneous Type 1 Diabetes Mellitus, Type 2 Diabetes Mellitus, and other types of insulin-dependent diabetes mellitus, or impaired glucose tolerance.

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Salt Lake City, UT, United States of America

Anna L Gooch