Corning Incorporated

Human Genome Sciences, Inc.

Ann MeeJin Ferrie

Ye Fang

Vasiliy Nikolaevich Goral

Craig A Rosen

Steven M Ruben

Paul E Young

John M Greene

Jian Ni

Guo-Liang Yu

Henrik S Olsen

Reinhard Ebner

Daniel R Soppet

Yue Zhou

Po Ki Yuen

Norman Henry Fontaine

Brian L Webb

Laurie A Brewer

Yulong Hong

Carrie L Fischer

David Henry

Joydeep Lahiri

Yanggu Shi

Jing-Shan Hu

Gregory A Endress

Ping Feng

Kimberly A Florence

Kenneth C Carter

Corinne Walerack

Lori E Romeo

Paul A Moore

Qi Wu

Yi Li

Roxanne Duan

Martial Hervy

Zhizhen Zeng

Hla Kyaw

Ying-Fei Wei

David William Lafleur

Jinlin Peng

Daniel P Bednarik

Lingyan Wang

Yan Jin

Sadashiva K Pai

Reiner L Gentz

Vitor Marino Schneider

Patrick J Dillon

Haodong Li

Allison Jean Tanner

Eric John Mozdy

Gregg A Hastings

Shawn Michael O'Malley

Glen Bennett Cook

Stephen Joseph Caracci

Anthony Glenn Frutos

Ying Wei

Ping Fan

Elizabeth Tran

Jeffrey Glenn Lynn

Charles Florence

Oberon Denaci Deichmann

Haiyan Sun

Chuan-Che Wang

Daniel P Soppet

Daniel R Bednarik

Haibei Hu

Fouad Janat

A cell culture apparatus may include a substrate defining a well. The well may define an interior surface, an exterior surface, an upper aperture and a nadir. The substrate may define a thickness between the interior and exterior surfaces that has a thickness proximate the nadir that is greater than or equal to a thickness proximate the upper aperture.

Microwell design and fabrication for generation of cell culture aggregates

A bioreactor system is provided that includes a cell culture vessel having a first end, a second end, and at least one reservoir between the first and second ends; and a cell culture matrix disposed in the at least one reservoir. The cell culture matrix has a structurally defined substrate with a surface for adhering cells thereto. The bioreactor system flows material through the at least one reservoir and through the cell culture matrix in a flow direction from the first end to the second end, and the cell culture matrix exhibits isotropic fluid flow permeability therethrough.

Packed-bed bioreactor systems and methods of using the same

A cell culture article includes a substrate having a polymer coating that is conducive to colony passaging of cells cultured on the coating. Example polymer coatings are formed from polygalacturonic acid (PGA), alginate, or combinations thereof. Cells cultured on the polymer coating can be separated from the substrate as a colony or layer of cells by exposing the polymer coating to (i) a chelating agent, (ii) a proteinase-free enzyme, or (iii) a chelating agent and a proteinase-free enzyme.

Proteinase-free coatings for colony passaging

A fixed-bed bioreactor system is provided that includes a vessel with a media inlet, a media outlet, and an interior cavity disposed between and in fluid communication with the media inlet and media outlet. The vessel further includes a cell culture substrate disposed in the interior cavity between the media inlet and the media outlet in a packed-bed configuration, the cell culture substrate including a plurality of porous disks in a stacked arrangement. The interior cavity includes a cell culture section and a spacer section, the cell culture substrate defining the cell culture section and the spacer section being disposed between the cell culture section and the media outlet, and each of the plurality of porous disks has a surface configured to culture cells thereon.

Fixed bed bioreactor and methods of using the same

A cell culture matrix is provided that has a substrate with a first side, a second side opposite the first side, a thickness separating the first side and the second side, and a plurality of openings formed in the substrate and passing through the thickness of the substrate. The plurality of openings allow flow of at least one of cell culture media, cells, or cell products through the thickness of the substrate, and provides a uniform, efficient, and scalable matrix for cell seeding, proliferation, and culturing. The substrate can be formed from a woven polymer mesh material that provides a high surface area to volume ratio for cells and good fluid flow through the matrix. Bioreactor systems incorporating the cell culture matrix and related methods are also provided.

A cell culture matrix for a perfusion-flow fixed-bed reactor is provided. The cell culture matrix includes a substrate having a porous sheet for adhering cells thereto. The sheet has a first side, a second side opposite the first side, a thickness separating the first side and the second side, and a plurality of openings formed in the substrate, arrayed in a regular pattern, and passing through the thickness of the substrate. The porous sheet is wound into a cylindrical shape having a plurality of wound layers, and the cell culture matrix does not include a spacer material or physical barrier between the plurality of wound layers of the substrate.

Woven cell culture substrates, bioreactor systems using the same, and related methods

Magnetic microcarrier beads have a particle size of 1 to 1000 micrometers and include a composite core and a polymer coating that surrounds and encapsulates the core. The composite core includes magnetic particles embedded within an indigestible polymer matrix. The coating is a digestible or indigestible polymer that facilitates cell adhesion to the surface of the beads during cell culture. Magnetic force can be used to agitate the microcarrier beads during cell culture as well as to separate the beads from cultured cells or processed bio-media.

Magnetic microcarriers

A packed-bed bioreactor system is provided, the system including a cell culture vessel having a first end, a second end, and a reservoir between the first and second ends; and a cell culture matrix disposed in the reservoir. The cell culture matrix includes a structurally defined substrate with a plurality of interwoven fibers having surfaces for adhering cells thereto. The substrate is disposed within the reservoir in a wound configuration creating a plurality of layers of substrate in the wound configuration, and none of the plurality of layers of substrate are separated by a spacer material.

A cell culture vessel () has walls and a substrate having a plurality of microcavities (), where each microcavity of the plurality of microcavities includes a concave well and an opening to allow the microcavity to be filled with liquid. A flange () surrounds the substrate having an array of microcavities. A channel () surrounds the flange, providing a moat around the microcavity substrate. The flange is angled. Methods of culturing cells in the cell culture vessel are also provided.

Cell culture vessel for 3D culture and methods of culturing 3D cells

A packed-bed bioreactor system for culturing cells is provided, the system including a cell culture vessel having at least one interior reservoir, an inlet fluidly connected to the reservoir, and an outlet fluidly connected to the reservoir; and a cell culture matrix disposed in the reservoir. The cell culture matrix includes a structurally defined multi-layered substrate for adhering cells thereto, and each layer of the multi-layered substrate has a physical structure and a porosity that are substantially regular and uniform.

Growing community of inventors

Painted Post, NY, United States of America

Ann MeeJin Ferrie