Zumutor Biologics Inc.

Ankurina Sharma

Maloy Ghosh

Bharath Ravindra Shenoy

Vivek Halan

Sunit Maity

Sanghamitra Bhattacharjee

Kavitha Iyer Rodrigues

Sahana Bhima Rao

Aswini Thanigaivel

Bhargav Prasad

Anuradha Hora

Sohang Chatterjee

Sankaranarayanan Srinivasan

Karthika Nair

Divya Unnikrishnan

Ashvini Kumar Dubey

Yogendra Manjunath Bangalore Muniraju

Sathyabalan Murugesan

Bairavabalakumar Natarajan

Subhra Prakash Chakrabarty

Veeresha Kamanagowda

Amir Khan

Pravin Kumar Dakshinamurthy

Pavithra Mukunda

Amol Maliwalave

Anurag Tiwari

Santosh Kumar

Shivani Patel

Rashmi Sharma

Nikitha Markanda

Jahnabi Hazarika

Nikitha M

Maliwalave Amol

Yogendra Manjunath Muniraju

Sankarnarayanan Srinivasan

The present disclosure relates to vectors for cloning and expressing genetic material including but not limiting to antibody gene or parts thereof and methods of generating said vectors. Said vectors express the antibody genes in different formats such as Fab or scFv as a part of intertransfer system, intratransfer system or direct cloning and expression in individual display systems. In particular, phage display technology is used to clone and screen potential antibody genes in phagemid which is followed by the transfer of said genes to yeast vector for further screening and identification of lead molecules against antigens. The present vectors have numerous advantages including uniquely designed inserts/expression cassettes resulting in efficient and smooth transfer of clonal population from phage to yeast vectors resulting in efficient library preparation and identification of lead molecules.

Vectors for cloning and expression of proteins, methods and applications thereof

The present disclosure relates to methods of obtaining cell with disrupted fucosylation and obtaining afucosylated protein. The present disclosure employs the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) technology in a protein producing cell line to produce afucosylated protein. The resulting protein, specifically the resulting monoclonal antibody is completely afucosylated and reveals higher degree of antibody dependent cellular cytotoxicity.

Afucosylated protein, cell expressing said protein and associated methods

The present disclosure relates to a method of generating an antibody library, not limiting to a synthetic antibody gene expression library built on pool of consensus nucleic acid sequences by using codon replacement technology. The present disclosure also relates to a synthetic antibody library generated by employing the method of the present disclosure and application(s) of said antibody library.

Method of generating a synthetic antibody library, said library and application(s) thereof

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Bengaluru, India

Ankurina Sharma