Gen-probe, Inc.

Ankur Shah

Amber Hillius

Mehrdad R Majlessi

Daniel Kolk

Pamela Douglass

Meghna Yadav

David Opalsky

Jason F Rhubottom

Paul M Darby

Barbara Lynn Eaton

James T Tuggle

Matthias Jost

Michael J Gilly

Jennifer L Tidd

Jo Ann Jackson

Siobhan Miick

Marcela Alejandra Carvallo Pinto

Hee Cheol Kim

Jian Yu Fung

Andrew S Gilder

Ann-Laure Shapiro

Alberto A Lio

Timothy J Scheer

David Aaron Buse

Disclosed are oligonucleotides, oligonucleotide compositions, kits, methods, formulations, and reaction mixtures that provide for sensitive and specific detection of a target nucleic acid sequence, or amplicon generated from a target nucleic acid sequence, of Varicella-Zoster Virus (VZV1 (if present) in a sample. The oligonucleotides, compositions, kits, methods, formulations, and reaction mixtures can be used to detect the presence of VZV in a sample. The oligonucleotides, compositions, kits, methods, formulations, and reaction mixtures can also be used to amplify specific target nucleic acid regions of VZV.

Compositions and methods for amplifying or detecting varicella-zoster virus

The disclosed disclosure is related to methods, compositions, and kits for targeting Adenovirus, Metapneumovirus, and/or Rhinovirus nucleic acid. Compositions include amplification oligomers and/or detection probe oligomers. Kits and methods comprise at least one of these oligomers. Methods include uniplex and multiplex amplification and detection reactions.

Compositions and methods to detect adenovirus nucleic acids and metapneumovirus and/or rhinovirus nucleic acids

Oligomer nucleotides, compositions, methods, kits, and uses are provided for detecting or quantifying a Human Cytomegalovirus virus 1 (CMV (human herpesvirus 5, HHV5) nucleic acid, e.g., using nucleic acid amplification and hybridization assays. Multiphase amplification of a CMV target sequence is also described. The oligomer nucleotides, compositions, methods, kits, and uses can be used to amplify and/or detect the UL56 gene of CMV.

Compositions and methods for amplifying, detecting or quantifying human cytomegalovirus

The disclosure is related to compositions, for targeting Adenovirus nucleic acid. Compositions include amplification oligomers and/or detection probe oligomers.

Compositions to detect adenovirus nucleic acids

A multiplexed nucleic acid amplification and detection system useful for detecting the presence of multiple specific nucleic acid sequences or single nucleotide polymorphisms (i.e., 'SNPs') in a temperature-dependent fashion using only a single fluorescence detection channel of a nucleic acid analyzer. The technique can be carried out using standard PCR instrumentation equipped for fluorescence detection or monitoring.

Temperature-selectable fret cassette signaling

Oligomer nucleotides, compositions, methods, kits, and uses are provided for detecting or quantifying a human polyomavirus BK virus (BKV) nucleic acid, e.g., using nucleic acid amplification and hybridization assays.

Compositions and methods for amplifying, detecting or quantifying human polyomavirus BK virus

Method of preparing a biological sample appropriate for use in a subsequent in vitro nucleic acid amplification reaction. A biological sample is combined with an alkaline composition that lyses cells and denatures DNA to create a first liquid composition. The first liquid composition is then mixed with a buffer, a detergent, and a solid support that captures DNA to create a second liquid composition. The buffer, detergent, and solid support can be delivered as components of a single reagent. Captured DNA strands can be used as templates in subsequently performed nucleic acid amplification and detection reactions with improved sensitivity.

Sample preparation method and system

The disclosed disclosure is related to methods, compositions, and kits for targeting Rhinovirus nucleic acid. Compositions include amplification oligomers and/or detection probe oligomers. Kits and methods comprise at least one of these oligomers. Methods include uniplex and multiplex amplification and detection reactions.

Methods to detect rhinovirus nucleic acids

Disclosed are compositions, methods, and kits that can be used to Epstein-Ban virus (EBV) in a sample undergoing testing. Nucleic acids of EBV can be isolated, amplified and detected with specificity by real-time PCR, and without interference from non-EBV organisms. In some embodiments, nucleic acids used for amplification are isolated from human blood, or blood products. Nucleic acid isolation, amplification and detection steps can all be carried out using an automated instrument.

Compositions and methods for detecting nucleic acids of Epstein-Barr virus

The disclosed disclosure is related to methods, compositions, and kits for targeting Adenovirus, nucleic acid. Compositions include amplification oligomers and/or detection probe oligomers. Kits and methods comprise at least one of these oligomers. Methods include uniplex and multiplex amplification and detection reactions.

Growing community of inventors

San Diego, CA, United States of America

Ankur Shah