Biocon Limited

Biocon Research Limited

Mylan Gmbh

Ankur Bhatnagar

Saravanan Desan

Ramakrishnan Melarkode

Harish Iyer

Jose Enrique Montero Casimiro

Laxmi Adhikary

Pradip Nair

Anuj Goel

Dinesh Baskar

Harish Venkatraman Pai

Kriti Shukla

Ruchika Srivastava

Sneha Lakshmandas Hemdev

Sunaina Prabhu

Rasika Venkataraman

Vana Raja

Lavanya Rao

The present invention relates to a method or process for controlling, inhibiting or reducing protein fucosylation in a eukaryote and/or eukaryotic protein expression system. Said method comprises carrying out the protein expression and/or post-translational modification in the presence of an elevated total concentration of manganese or manganese ions.

Methods for controlling fucosylation levels in proteins

The present invention relates to a method or process for controlling, inhibiting or reducing protein fucosylation in a eukaryote and/or a eukaryotic protein expression system. Said method comprises carrying out the protein expression and/or post-translational modification in the presence of an elevated total concentration of manganese or manganese ions.

The present disclosure relates to methods of decreasing lactate production in cell culture using divalent transitional metallic salts. The present disclosure also relates to a method of producing polypeptide by adding divalent transitional metallic salt to the cell culture medium for reducing lactate accumulation followed by fermenting and recovering the polypeptide.

Methods for reducing accumulation of lactate during culturing and method for producing polypeptide

The present disclosure relates to a method of reducing heterogeneity in antibodies during culturing, wherein the heterogeneity is due to proportion of charge variant of the antibody. The disclosure also comprises a process of growing cells in a cell culture system that results in antibodies with the reduced heterogeneity. In one embodiment antibody heterogeneity is reduced by the addition of divalent transitional metal ions such as zinc (Zn) to the cell culture medium. In another embodiment antibody heterogeneity is reduced by decreasing the osmolality of the cell culture medium.

Method for reducing heterogeneity of antibodies and a process of producing the antibodies thereof

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Bengaluru, India

Ankur Bhatnagar