University of Calcutta

Anjan Kr Dasgupta

Sarita Roy

Arka Mukhopadhyay

Tamoghna Bhattacharyya

Nalok Dutta

Krishanu Chakraborti

Krishanu Chakraborty

Sufi Oasim Raja

Hirak Kumar Patra

Dhrubajyoti Chattopadhyay

Santiswarup Singha

A technique for determining whether or not a fluorescent material exhibits a directionally dependent property, such as anisotropy or chirality, involves illuminating the particle at its excitation wavelength to stimulate fluorescent emission at both a full-frequency (fundamental) wavelength and a half-frequency wavelength. The ratio of the full-frequency signal strength to the half-frequency signal strength provides an indication of the sample's directionally dependent property. This half-frequency spectral analysis can be used to sort anisotropic particles suspended in fluid flowing through a flow cytometer. For instance, the present technique may be used to separate racemic mixtures of chiral enantiomers of cells, pharmaceutical compounds, and other samples.

Half-frequency spectral signatures

Methods including contacting, in the solid state, a sample comprising a first organic molecule with a composition comprising: a carbon nanotube, such that an interface forms between the sample and the composition; observing any movement of the interface; and characterizing the first organic molecule based on the observed movement are described.

Molecular discriminators using carbon nanotubes

Enzyme compositions with enhanced enzyme activity and/or thermophilic and psychrophilic stability are described. Additionally, methods and kits for making and using the enzyme compositions are provided.

Psychrophilic enzymes compositions and methods for making and using same

The present technology provides liquid quality assessment systems and methods for their preparation and use. The systems can include a light source configured to illuminate a liquid sample, a reflecting surface configured to reflect light scattered by the liquid sample, and a detector configured to detect light intensity, wherein the light source illuminates the liquid sample with a first incident light when the reflecting surface is absent; the detector detects a first light scattered by the liquid sample in response to the first incident light; the light source illuminates the liquid sample with a second incident light when the reflecting surface is present; and the detector detects a second light which is a combination of light scattered by the liquid sample in response to the second incident light and light reflected by the reflecting surface of light scattered by the liquid sample in response to the second incident light.

Systems and methods for liquid quality assessment

A method of determining the relative concentrations of enantiomeric forms of a compound in an enantiomeric mixture includes combining the enantiomeric mixture with carbon nanotubes or graphene to form a carbon-enantiomer mixture, exposing the mixture to a monochromatic polarized light, and analyzing reflected polarized light from the mixture using a differential analyzer.

Configurational chirality based separation

Provided herein are compositions and methods for enhancing enzyme activity, half-life, thermostability, enhanced activity at elevated temperature, and/or decreasing the pH dependency of laccase enzyme. Also provided are methods for using the composition comprising the stabilized enzymes.

Stabilized laccase enzyme and methods of making and using the same

Exposure to a static magnetic field changes the fluorescence intensity of a wide range of fluorophores, including small molecules (e.g., tryptophan), complex organizations of fluorophores (e.g., proteins), quantum dots, nanoparticles, and other materials. Different materials may experience different changes in fluorescence emission upon exposure to a magnetic field&#x2014;for instance, some or all of a material's fluorescence emission spectrum may increase in amplitude or shift in wavelength. Different materials may also experience different changes in relaxation time, which is the time constant associated with fluorescence decay. These magnetically induced differences fluorescence emission spectra and decay can be used to identify, classify, or sort materials noninvasively.

Static magnetic field induced differential fluorescence emission

A method includes detecting DO in a sample by fluorescence spectroscopy. The fluorescence spectroscopy may be near-infrared fluorescence spectroscopy. The method may include observing an excitation wavelength of the sample at 620 nm to 640 nm. The method may also include observing an emission wavelength of the sample at 900 nm to 1000 nm. The method includes detecting DO and DO nano-clusters and their alterations in presence of suspended or colloidal objects including bio-molecules or cells, by emission spectroscopy.

NIR fluorescence of heavy water

The present technology provides for enzyme compositions with enhanced enzyme activity, thermophilic and psychrophilic stability.

Enzyme compositions

Provided herein are compositions and methods for enhancing enzyme activity, half-life and/or thermostability. Also provided herein are compositions and methods including the enhanced enzymes. Also provided herein are methods and compositions related to improved pectinolytic enzymes, such as pectate lyase, which exhibit enhanced activity, thermostability and/or longer half-life.

Thermostable enzymes and methods of making and using the same

Technologies are generally described for providing and using a material including: a template molecule; a first cluster of one or more nanoparticles located at a first site on the template molecule; and a second cluster of one or more nanoparticles located at a second site on the template molecule and spaced apart from the first cluster. In some embodiments, the first and second clusters of nanoparticles exhibit a plasmon resonance having a first resonant peak and a second resonant peak.

Nano sensing of temperature using equal intensity double plasmon resonance (EIDPR)

Technologies are generally described for systems and methods for detecting chiral properties of materials and separating materials based on their chiral properties. A chiral vector is constructed from anisotropy properties of a polarization-dependent output signal from a sample. Different types of molecules from the sample can be differentiated based on a magnitude of the chiral vector. Chiral properties of the sample can be detected based on an angle of the chiral vector. The output signal can be a fluorescent emission from the sample and can be used to detect chiral properties of a substantially opaque sample.

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Kolkata, India

Anjan Kr Dasgupta