Mitsui Chemicals, Inc.

Anjali Madhavan

Tomonori Hidesaki

Ryota Fujii

Su Sun Chong

Yoshiko Matsumoto

Hiroshi Shimizu

Hitoshi Takahashi

Tadashi Araki

Mitsufumi Wada

Takashi Morishige

Nozomi Takebayashi

Tomokazu Shirai

Koh Amano

Chikara Furusawa

Takashi Hirasawa

Toshihiro Tateno

Ayako Endo

Dominik Lukas Jürgen-Lohmann

Disclosed is an acetyl-CoA-producing microorganism, which is obtained by imparting malate thiokinase and malyl-CoA lyase enzymatic activities to a microorganism having none of the following (a), (b), (c) or (d), without imparting any of (a), (b), (c) or (d), or, even when one or more of (a), (b), (c) or (d) are imparted, not allowing the functions thereof to be exerted: (a) a carbon dioxide fixation cycle having an enzymatic reaction from malonyl-CoA to malonate semialdehyde or 3-hydroxypropionate, (b) a carbon dioxide fixation cycle having an enzymatic reaction from acetyl-CoA and COto pyruvate, (c) a carbon dioxide fixation cycle having an enzymatic reaction from crotonyl-CoA and COto ethylmalonyl-CoA or glutaconyl-CoA or (d) a carbon dioxide fixation cycle having an enzymatic reaction from COto formate.

Microorganism for production of chemicals derived from acetyl-CoA

An acetyl-CoA producing microorganism obtained by imparting at least one enzymatic activity selected from the group consisting of malate thiokinase, malyl-CoA lyase, glyoxylate carboligase, 2-hydroxy-3-oxopropionate reductase, and hydroxypyruvate reductase, to a microorganism that does not have any of the following (a), (b), (c), (d) or (e): (a) a carbon dioxide fixation cycle including an enzymatic reaction from malonyl-CoA to malonate semialdehyde or 3-hydroxypropionate; (b) a carbon dioxide fixation cycle including an enzymatic reaction from acetyl-CoA and COto pyruvate; (c) a carbon dioxide fixation cycle including an enzymatic reaction from crotonyl-CoA and COto ethylmalonyl-CoA or glutaconyl-CoA; (d) a carbon dioxide fixation cycle including an enzymatic reaction from COto formate; or (e) at least one selected from the group consisting of malate thiokinase and malyl-CoA lyase.

Microorganism having carbon dioxide fixation cycle introduced thereinto

An acetyl-CoA-producing microorganism, which is capable of efficiently synthesizing acetyl-CoA using carbon dioxide, and a substance production method using the same are provided. An acetyl-CoA-producing microorganism including an acetyl-CoA production cycle obtained by imparting at least one type of enzymatic activity selected from the group consisting of malate thiokinase, malyl-CoA lyase, glyoxylate carboligase, 2-hydroxy-3-oxopropionate reductase, and hydroxypyruvate reductase, to a microorganism.

Microorganism having carbon dioxide fixation pathway introduced thereinto

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Singapore, Singapore

Anjali Madhavan