Alcon Research, Ltd.

Thermo Fisher Scientific Gmbh

Dharmacon, Inc.

Arrowhead Research Corporation

Thermo Fisher Scientific Biosciences Inc.

Anja Smith

Angela Reynolds

Jon E Chatterton

Xinyu Zhang

Anastasia Khvorova

Devin Leake

Annaleen Vermeulen

Barbara Robertson

Scott Baskerville

Vascular endothelial growth factor A (VEGFA) is a chemical signal produced by cells that stimulates the growth of new blood vessels, and overexpression of VEGFA can lead to undesirable physiological conditions. Through the identification of new siRNA and modifications that improve the silencing ability of these siRNA in vivo, therapeutic compositions and methods have been invented to address the problems associated with this overexpression.

siRNA targeting VEGFA and methods for treatment in vivo

The disclosure provides methods for inhibiting the activity of a miRNA cluster in a cell, and also for screening a cell for a phenotype(s) of interest resulting from inhibition of a miRNA cluster. The methods use a cluster pool which comprises at least one miRNA inhibitor specific for each miRNA in the miRNA cluster. MiRNA inhibitors are described that induce apoptosis in breast cancer cells and hence are useful in the treatment of breast cancer. The disclosure also provides pharmaceutical compositions which are useful for the treatment of breast cancer; methods for inducing the nuclear translocation of NF-&#x3ba;B in a breast cancer cell; methods for inducing the nuclear translocation of c-Jun in a breast cancer cell; method for inhibiting the nuclear translocation of NF-&#x3ba;B in a breast cancer cell; and methods for providing prognostic medical information relating to breast cancer progression.

Screening of micro-RNA cluster inhibitor pools

Growing community of inventors

Thornton, CO, United States of America

Anja Smith