Danisco A/s

Genencor International, Inc.

Dupont Nutrition Biosciences Aps

Anja Hemmingen Kellet-Smith

Andrew Shaw

Karsten Matthias Kragh

Carol Marie Fioresi

Wei Liu

Gijsbert Gerritse

Charlotte Refdahl Thoudahl

Casper Tune Berg

Bo Spange Sørensen

Patrick M F Derkx

Patrick Maria Franciscus Derkx

Variant polypeptides derivable from a parent polypeptide having non-maltogenic exoamylase activity, in which the variant polypeptides comprise an amino acid mutation at one or more positions selected from the group consisting of: 121, 161, 223, 146, 157, 158, 198, 229, 303, 306, 309, 316, 353, 26, 70, 145, 188, 272, 339, with reference to the position numbering of aexoamylase sequence shown as SEQ ID NO: 1.

Modified amylases from pseudomonas species, methods of making and uses thereof

The invention describes a PS4 variant polypeptide derivable from a parent polypeptide having amylase activity selected from the group consisting of: (a) a polypeptide comprising an amino acid mutation at each of positions 33, 34, 121, 134, 141, 146, 157, 161, 178, 179, 223, 229, 272, 303, 307, 309 and 334; (b) a polypeptide comprising an amino acid mutation at each of positions 33, 34, 121, 134, 141, 145, 146, 157, 178, 179, 223, 229, 272, 303, 307 and 334; (c) a polypeptide comprising an amino acid mutation at each of positions 33, 34, 121, 134, 141, 146, 157, 178, 179, 223, 229, 272, 303, 307, 309 and 334; and (d) a polypeptide comprising an amino acid mutation at each of positions 3, 33, 34, 70, 121, 134, 141, 146, 157, 178, 179, 223, 229, 272, 303, 307, 309 and 334; with reference to the position numbering of aexoamylase sequence shown as SEQ ID NO: 1, uses of such a polypeptide as a food or feed additive, and nucleic acids encoding such.

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Søborg, Denmark

Anja Hemmingen Kellet-Smith