Precision Nanosystems Ulc

The University of British Columbia

Global Life Sciences Solutions Canada Ulc

Precision Nanosystems Inc.

Anitha Thomas

Rebecca Anne Grace De Souza

Andrew William Brown

Andre Wild

Timothy Leaver

Robert James Taylor

Euan Ramsay

Colin Walsh

Kevin Ou

Aysha Ansari

Nikita Jain

R James Taylor

Eric Ouellet

Grace Tharmini Tharmarajah

Gesine Heuck

Jagbir Singh

Shyam Madhusudan Garg

Tara L Fernandez

Rebecca Anne De Souza

Euan Ramsey

The present document describes compounds, or pharmaceutically acceptable salt thereof, of a core formula (I) where Rfeatures an amine group, particularly useful in the formulation of lipid particles including nucleic acid therapeutic agents, or proteins, or both, and for delivery of nucleic acid and protein therapeutics to cells in vivo or ex vivo, including anticancer and vaccine applications.

Ionizable lipids for nucleic acid delivery

A reagent composition for delivery nucleic acid therapeutics to cells is provided. The reagent includes ionizable lipid, structural lipid, and a stabilizing agent which improves the transfection efficiency of nucleic acids, and leaves transfected cells viable. The 5 transfection reagent is effective in plasmid and mRNA delivery, and in neurons and progenitor-like cells. It may be used for ex vivo cell therapy.

Compositions for transfecting resistant cell types

A transfection reagent composition comprising: 30-60 MOL. % of a cationic lipid, or a pharmaceutical acceptable salt thereof; 10-60 MOL % of a structural lipid; 10-20 MOL % of a triglyceride; and 0.1 to about 10 MOL. % of a stabilizing agent, is provided. The transfection agent is effective in transfecting cells, particularly neurons, with siRNA, mRNA and plasmid nucleic acid, Sand maintaining viability of the cells as well as activity of the delivered nucleic acid.

The present disclosure is directed towards a disposable microfluidic cartridge configured for use in a system for the small scale production of nanoparticles used in scientific research or therapeutic applications. The system can be used to produce a wide variety of nanoparticles, including but not limited to lipid and polymer nanoparticles, carrying a variety of payloads. The system provides for a simple workflow which in certain embodiments can be used to produce a sterile product.

Disposable microfluidic cartridge

Growing community of inventors

New Westminster, Canada

Anitha Thomas