Unversität Zürich

Technische Universiteit Eindhoven

Universitaet Zuerich

Anita Driessen-Mol

Franciscus Petrus Thomas Baaijens

Simon Philipp Hoerstrup

Frank Baaijens

Bart Sanders

Simon-Philipp Hoerstrup

Sandra Vossen-Loerakker

Maximilian Y Emmert

The present invention concerns a tissue-engineered medical device, as well as a method for the production said medical device, comprising the following steps: providing a polymer scaffold comprising a mesh comprising polyglycolic acid, and a coating comprising poly-4-hydroxybutyrate; application of a cell suspension containing preferably human cells to the polymer scaffold; placement of the seeded polymer scaffold in a bioreactor and mechanical stimulation by exposure to a pulsatile flux of incremental intensity, thereby forming an extracellular matrix; mounting of the graft on a conduit stabilizer and incubation in cell culture medium; decellularisation of the graft in a washing solution; nuclease treatment of the graft; and rinsing of graft. The invention further comprises and various steps of quality control of the tissue-engineered medical device.

Tissue-engineered medical device

Various inserts, called shapers and spacers, are provided for controlling tissue engineered heart valve (TEHV) leaflet geometry during culture. These inserts will prevent TEHV leaflet retraction during culture, be able to control the leaflet geometry during culture, enable culturing TEHV leaflets with a larger coaptation area, control the height of the coaptation area, maintain TEHV leaflet curvatures, and/or enable possibilities to culture TEHV leaflets in open configuration.

Controlling tissue engineered heart valve geometry by using predefined inserts during culture

Growing community of inventors

JM Rosmalen, Netherlands

Anita Driessen-Mol