Harvard College

Anik Debnath

George M Church

Henry Hung-yi Lee

Javier Fernandez Juarez

Provided herein, in some embodiments, are artificial secretion peptides capable of directing secretion fromfor use, for example, in producing heterologous proteins, including therapeutic proteins.

Artificial secretion peptides for heterologous protein production

The disclosure provides methods of making a tetracycline inducible expression system in a cell. The methods include providing the cell with a first nucleic acid sequence comprising a first promoter operably linked to a tetracycline repressor gene coding sequence, providing the cell with a second nucleic acid sequence comprising a second promoter operably linked to a coding sequence of a gene of interest wherein the second promoter is modified to include one or more tetracycline repressor protein binding sites, and determining the expression of the gene of interest in the presence or absence of tetracycline. The disclosure further provides nucleic acid sequences, vectors and cells including the tetracycline inducible modified promoter.

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Boston, MA, United States of America

Anik Debnath