Hoffmann-la Roche Inc.

Ventana Medical Systems, Inc.

F. Hoffmann-la Roche Ag

Angelika Fuchs

Srinivas Chukka

Gerardo Fernandez

Quan Yuan

Elizabeth Little

Jan Kuentzer

Otto Huber

Alex Kohn

Franziska Mech

Eckhart Guthoehrlein

An image analysis method for analyzing an IHC tissue sample includes storing, for each of one or more sets of digital images, a set-ID in a spatial database of a spatial DBMS, the images of each set depicting the same or adjacent layers of the IHC tissue sample; storing, for each of the, a plurality of spatial data objects in the database in association with the set-ID; creating, by the spatial DBMS, at least one spatial index covering the objects of the images contained in the one or more sets; applying, by the spatial DBMS, a spatial database operation on the at least one spatial index for determining the relative positions of objects of two or more images of a selected set; and providing the relative positions as input for determining the distance and/or relative position of the biomedical features of the tissue sample.

Spatial index creation for IHC image analysis

Systems and methods for analyzing vessels in multiplexed images include detecting large vessels using a spoke feature detection method, detecting long and narrow vessels using a line feature detection method, detecting smaller vessels using rolling-ball filtering and binary image operations to generate a mask, and evaluating any contour polygons resulting from these operations using quality measurements and other thresholds. Maturity determination and nuclei detection are also performed, resulting in an output of vessel characteristics and co-locations enabling enhanced analysis of multispectral images.

Vessel analysis in multiplexed images

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Munich, Germany

Angelika Fuchs