Isoplexis Corporation

Qiagen Sciences, LLC

Angela DeLucia

Jerzy Olejnik

Cheng-Yao Chen

Mong Sano Marma

Luisa Andruzzi

Dona Hevroni

Ben McNally

John Andrew Sheridan

Austin Ricker

Timothy Pelletier

Jimmy Adediran

Thomas William Schoenfeld

Elizabeth Carpenter

Magnus Besev

Ryan Charles Heller

The present invention relates to a polymerase enzyme from 9° N with improved ability to incorporate reversibly terminating nucleotides. The enzyme comprising mutations in the motif A region. The invention also relates to methods of using such enzymes as well as a kit with such polymerases.

Polymerase enzyme from 9°N

The present invention provides methods, compositions, mixtures and kits utilizing 5-Chloro-2-methyl-4-isothiazolin-3-one in sequencing reactions, and in particular, sequencing reactions where deoxynucleoside triphosphates comprising a 3'-O position capped by a disulfide-based 3&#x2032;-terminator group are used. In one embodiment, the deoxynucleoside triphosphates comprise a 3&#x2032;-O position capped by a group comprising methylenedisulfide as a cleavable protecting group and a detectable label reversibly connected to the nucleobase of said deoxynucleoside. In addition, thiol-containing compounds and scavengers of thio-containing compounds are described. Such compounds provide new possibilities for future sequencing technologies, including but not limited to Sequencing by Synthesis.

DNA sequencing reaction additive

The present invention provides methods, compositions, mixtures and kits utilizing deoxynucleoside triphosphates comprising a 3'-O position capped by a group comprising methylenedisulfide as a cleavable protecting group and a detectable label reversibly connected to the nucleobase of said deoxynucleoside. In addition, thiol-containing compounds and scavengers of thio-containing compounds are described. Such compounds provide new possibilities for future sequencing technologies, including but not limited to Sequencing by Synthesis.

Thiol-containing cleave reagents and oxidative wash

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Cambridge, MA, United States of America

Angela DeLucia