Miles Inc.

Angela A Michaels

Ronald G Sommer

Paul F Corey

Michael J Pugia

James P Albarella

Michael Salvati

Lois J Proud

Robert W Trimmer

The detection of protein is accomplished using novel composition and method involving phenolsulfonephthalein protein error indicator, buffer and an aliphatic ether-polycarbonate present in an amount equal to or less than ten percent by weight.

Assay for the determination of protein in a biological sample

The present invention provides an analytical test strip for the detection of protein in a biological sample including a novel combination of protein error indicators. The invention includes, in combination, a partially halogenated phenolsulfonephthalein protein error indicator having nitro or nitroso substituent groups in the B and/or C rings and a merocyanine protein error indicator.

Merocyanine and nitro or nitroso substituted polyhalogenated

The present invention provides a novel group of protein error indicators including nitro or nitroso substituent groups in the B and C rings of a partially halogenated triphenylmethane compound.

Nitro or nitroso substituted polyhalogenated phenolsulfonephthaleins as

A screening method for the determination of 10.sup.4 gram negative bacteria per milliliter of an undiluted urine sample and a unitary screening test device for the determination of at least 10.sup.5 gram negative bacteria per milliliter of an undiluted urine sample. The method and test device make use of Limulus amebocyte lysate (LAL) and a synthetic substrate containing a chromogenic or fluorogenic leaving group capable of being cleaved by activated lysate. This screening method and unitary screening device provide a quick, convenient, inexpensive indication of the possible presence of a urinary tract infection caused by gram negative bacteria.

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Elkhart, IN, United States of America

Angela A Michaels