E.i. Dupont De Nemours and Company

Pioneer Hi-bred International, Inc.

André Abad

Ronald D Flannagan

Albert Laurence Lu

Billy Fred McCutchen

Rafael Herrmann

Hua Dong

Eric J Schepers

Janet Ann Rice

James F Wong

James Kevin Presnail

James F H Wong

Theodore W Kahn

Cao-Guo Yu

The invention providestoxins having pesticidal activity against insect pests, including Lepidoptera. Particular embodiments of the invention provide isolated pesticidal proteins and pesticidal compositions. These compositions find use in methods for controlling pests, especially plant pests.

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The invention provides nucleic acids, and variants and fragments thereof, obtained from strains ofencoding &#x3b4;-endotoxins having pesticidal activity against insect pests, including Lepidoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, expression cassettes, and transformed microorganisms and plants comprising a nucleic acid of the invention. These compositions find use in methods for controlling pests, especially plant pests.

The invention provides nucleic acids, and variants and fragments thereof, obtained from strains ofencoding Cry9 &#x3b4;-endotoxins having pesticidal activity against insect pests, including. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, expression cassettes, and transformed plants comprising a nucleic acid of the invention.

Compositions and methods for protecting a plant from an insect pest are provided. The invention provides mutagenized nucleic acids that have been engineered to encode pesticidal polypeptides having increased resistance to proteolytic degradation by a plant protease. In particular, nucleic acid sequences encoding pesticidal polypeptides modified to comprise a proteolytic protection site that confers resistance to degradation or proteolytic inactivation by a plant protease are provided. Particular embodiments of the invention provide expression cassettes and transformed plants, plant cells, and seeds. These compositions find use in methods for protecting a plant from a pest.

Genes encoding plant protease-resistant pesticidal proteins and method of their use

Compositions and methods for protecting a plant from an insect pest are provided. The invention provides mutagenized nucleic acids that have been engineered to encode pesticidal polypeptides having increased resistance to proteolytic degradation by a plant protease. In particular, nucleic acid sequences encoding pesticidal polypeptides modified to comprise a proteolytic protection site that confers resistance to degradation or proteolytic inactivation by a plant protease are provided. Particular embodiments of the invention provide pesticidal polypeptide compositions and formulations, expression cassettes, and transformed plants, plant cells, and seeds. These compositions find use in methods for controlling pests, especially plant pests. Novel plant proteases, sequences encoding these proteases, and methods for their use are also provided.

Genes encoding proteins with pesticidal activity

The invention provides nucleic acids, and variants and fragments thereof, obtained from strains ofencoding &#x3b4;-endotoxins having pesticidal activity against insect pests. The invention further provides mutagenized nucleic acids that have been modified to encode pesticidal polypeptides such as endotoxins having improved pesticidal activity and/or altered pest specificity. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins that may be optimized as well as pesticidal compositions, expression cassettes, and transformed microorganisms and plants comprising a nucleic acid of the invention. These compositions find use in methods for controlling pests, especially plant pests.

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W. Des Moines, IA, United States of America

André Abad