Harvard College

Children's Medical Center Corporation

Andries D Van Der Meer

Donald Elliot Ingber

Abhishek Jain

Daniel Levner

Christopher David Hinojosa

Riccardo Barrile

Andrew L Frelinger, Iii

Marinke Van Der Helm

Marjon Zamani

Alan David Michelson

Systems and methods for measuring dynamic hydraulic conductivity and permeability associated with a cell layer are disclosed. Some systems include a microfluidic device, one or more working-fluid reservoirs, and one or more fluid-resistance element. The microfluidic device includes a first microchannel, a second microchannel, and a barrier therebetween. The barrier includes a cell layer adhered thereto. The working fluids are delivered to the microfluidic device. The fluid-resistance elements are coupled to one or more of the fluid paths and provide fluidic resistance to cause a pressure drop across the fluid-resistance elements. Mass transfer occurs between the first microchannel and the second microchannel, which is indicative of the hydraulic conductivity and/or dynamic permeability associated with the cells.

Fluidic device for quantifying the dynamic permeability and hydraulic conductivity of living tissue layers

A method is directed to determining a thrombosis function and includes flowing a fluid sample over a surface having a fixed endothelial cell monolayer. The method further includes stimulating the fixed endothelial cell monolayer to induce formation of a clot, the clot being formed via interaction between the fixed endothelial cell monolayer and the fluid sample. In response to the clot formation, the method further includes determining a thrombosis function associated with the fluid sample and the fixed endothelial cell monolayer.

Methods, systems, and compositions for determining blood clot formation, and uses thereof

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Enschede, Netherlands

Andries D Van Der Meer