Sandia Corporation

Andrew Walker

Blake A Simmons

David Ingersoll

Joanne V Volponi

Disclosed is an apparatus and method for continuously converting sucrose to &#x3b2;-D-glucose. The method comprises a three-stage enzymatic reactor in which an aqueous solution of sucrose is first converted into a solution of fructose and &#x3b1;-D-glucose by passing it through a porous, packed column containing an inert media on which invertase is immobilized. This solution is then sent through a second packed column containing glucose isomerase and finally a third packed column containing mutarotase. Solution temperature and pH are adjusted to maximize glucose output.

Method for converting sucrose to &#x3b2;-D-glucose

Disclosed is an apparatus and method for continuously converting sucrose to &#x3b2;-D-glucose. The method comprises a three stage enzymatic reactor in which an aqueous solution of sucrose is first converted into a solution of fructose and &#x3b1;-D-glucose by passing it through a porous, packed column containing an inert media on which invertase is immobilized. This solution is then sent through a second packed column containing glucose isomerase and finally a third packed column containing mutarotase. Solution temperature and pH are adjusted to maximize glucose output.

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Woodinville, WA, United States of America

Andrew Walker