Berkeley Lights, Inc.

Formfactor, Inc.

Bruker Cellular Analysis, Inc.

Phenomex Inc.

Andrew W McFarland

J Tanner Nevill

Eric D Hobbs

Keith J Breinlinger

Randall D Lowe, Jr

Gang F Wang

Daniele Malleo

Mark P White

Kevin T Chapman

Kristin G Beaumont

Russell A Newstrom

Troy A Lionberger

Volker L S Kurz

Xiaohua Wang

Darcy K Kelly-Greene

Aathavan Karunakaran

Jason M McEwen

Xiao Guan

Natalie C Marks

Paul M Lebel

Brandon R Bruhn

Kai W Szeto

Nan-Linda Ding

Paul M Lundquist

Carl V Reynolds

Justin K Valley

Eric Sackmann

Yoichi Urakawa

Nobuhiro Kawamata

Keith Joseph Breinlinger

Kevin Youl Yasumura

Jonathan Cloud Dragon Hubbard

Eric K Sackmann

Incubators including an enclosure with an internal chamber configured to support a cell culture plate comprising a plurality of wells are disclosed. The enclosure includes a plurality of openings configured to allow access to the wells. The incubators include a sealing element configured to seal the plurality of openings in the enclosure. The sealing element comprises a plurality of openings corresponding to at least a subset of the plurality of openings in the enclosure. Access to the internal chamber can be provided by aligning the plurality of openings in the sealing element with the plurality of openings in the enclosure. Methods for using the incubators are also provided.

Well plate incubator

In situ-generated microfluidic isolation structures incorporating a solidified polymer network, methods of preparation and use, compositions and kits therefor are described. The ability to introduce in real time, a variety of isolating structures including pens and barriers offers improved methods of micro-object manipulation in microfluidic devices. The in situ-generated isolation structures may be permanently or temporarily installed.

In situ-generated microfluidic isolation structures, kits and methods of use thereof

Systems, methods and kits are described for culturing one or more biological cells in a microfluidic device, including provision of nutrients and gaseous components configured to enhance cell growth, viability, portability, or any combination thereof. In some embodiments, culturing a single cell may produce a clonal population in the microfluidic device.

Microfluidic cell culture

A method of processing and storing biological cells includes introducing a flowable medium into a microfluidic device, the flowable medium including biological cells; sequestering one or more biological cells from the flowable medium in one or more isolation regions of the microfluidic device; and freezing the microfluidic device including the one or more biological cells sequestered therein.

Freezing and archiving cells on a microfluidic device

Apparatuses and methods are described for the use of optically driven bubble, convective and displacing fluidic flow to provide motive force in microfluidic devices. Alternative motive modalities are useful to selectively dislodge and displace micro-objects, including biological cells, from a variety of locations within the enclosure of a microfluidic device.

Microfluidic devices for optically-driven convection and displacement, kits and methods thereof

Incubators are disclosed which include an enclosure with an internal chamber configured to support a cell culture plate and provide an environment suitable for maintaining and/or culturing biological cells. The enclosure can include one or more openings configured to allow access to the cell culture plate. The incubators can further include a structure having a plurality of openings configured to be aligned with a corresponding plurality of wells in the cell culture plate, and a sealing element configured to moveably seal the plurality of openings in the structure. The sealing element can comprise a plurality of openings corresponding to at least a subset of the plurality of openings of the structure. Access to the internal chamber can be provided by aligning the plurality of openings in the sealing element with the plurality of openings in the structure. Methods for using the incubators are also provided.

Well-plate incubator

A microfluidic device can comprise a plurality of interconnected microfluidic elements. A plurality of actuators can be positioned abutting, immediately adjacent to, and/or attached to deformable surfaces of the microfluidic elements. The actuators can be selectively actuated and de-actuated to create directed flows of a fluidic medium in the microfluidic (or nanofluidic) device. Further, the actuators can be selectively actuated and de-actuated to create localized flows of a fluidic medium in the microfluidic device to move reagents and/or micro-objects in the microfluidic device.

Actuated microfluidic structures for directed flow in a microfluidic device and methods of use thereof

Growing community of inventors

Berkeley, CA, United States of America

Andrew W McFarland