Roche Sequencing Solutions, Inc.

Genia Technologies, Inc.

Roche Molecular Systems, Inc.

Other

Roche Diagnostics Gmbh

F. Hoffmann-la Roche Ag

Andrew Trans

Randall W Davis

Edward Shian Liu

Eric Takeshi Harada

Anne Aguirre

James Pollard

Cynthia Ann Cech

Dmitriy Gremyachinskiy

Hui Tian

Frank Bergmann

Christoph Seidel

John S Foster

Roger Chen

Robert A Yuan

Peter Crisalli

Hannes Kuchelmeister

Ashwini Bhat

Lars Hillringhaus

Cynthia Cech

Peter J Crisalli

This disclosure provides a biochip comprising a plurality of wells. The biochip includes a membrane that is disposed in or adjacent to an individual well of the plurality of wells. The membrane comprises a nanopore, and the individual well comprises an electrode that detects a signal upon ionic flow through the pore in response to a species passing through or adjacent to the nanopore. The electrode can be a non-sacrificial electrode. A lipid bilayer can be formed over the plurality of wells using a bubble.

Methods for forming lipid bilayers on biochips

The present disclosure relates to compositions and methods based on polypeptide-tagged nucleotide, and the use of such polypeptide-tagged nucleotides in nanopore devices and methods.

Polypeptide tagged nucleotides and use thereof in nucleic acid sequencing by nanopore detection

The present disclosure relates to compounds comprising a negatively-charged polymer moiety which is capable of entering a nanopore and upon entering a nanopore in the presence of positive ions results in an increased flow of the positive ions through the nanopore. The present disclosure provides methods of preparing the compounds and for their use as nanopore-detectable tags, in particular, for nanopore-based nucleic acid detection and sequencing.

Tagged nucleotides useful for nanopore detection

A biochip for molecular detection and sensing is disclosed. The biochip includes a substrate. The biochip includes a plurality of discrete sites formed on the substrate having a density of greater than five hundred wells per square millimeter. Each discrete site includes sidewalls disposed on the substrate to form a well. Each discrete site includes an electrode disposed at the bottom of the well. In some embodiments, the wells are formed such that cross-talk between the wells is reduced. In some embodiments, the electrodes disposed at the bottom of the wells are organized into groups of electrodes, wherein each group of electrodes shares a common counter electrode. In some embodiments, the electrode disposed at the bottom of the well has a dedicated counter electrode. In some embodiments, surfaces of the sidewalls are silanized such that the surfaces facilitate the forming of a membrane in or adjacent to the well.

Process for biosensor well formation

Growing community of inventors

Mountain View, CA, United States of America

Andrew Trans